Production of a new monoclonal antibody specific to human des-gamma-carboxyprothrombin in the presence of calcium ions. Application to the development of a sensitive ELISA-test

J Immunoassay. 1995 May;16(2):213-29. doi: 10.1080/15321819508013559.

Abstract

In order to explore pathologies possibly associated with vitamin K deficiency, several monoclonal antibodies (mAbs) were produced against human Desgamma-Carboxy-Prothrombin (DCP). One of these mAbs, designated C4B6, detected DCP forms in the presence of Calcium ions, confirmed by comparison with the patterns of two electrophoretic techniques: Affino-Immuno-Electrophoresis (CAIE) and Polyacrylamide Gel Electrophoresis followed by Electro-blotting (PAGE-Blot). An Enzyme-Linked-Imunosorbent Assay (ELISA) using mAb C4B6 has been developed, optimized and standardized. It has proven to be specific for DCP forms and has a minimum sensitivity of 0.156 A.U/ml.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / drug effects
  • Biomarkers*
  • Calcium*
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Humans
  • Immunoblotting
  • Immunoelectrophoresis
  • Mice
  • Mice, Inbred BALB C
  • Protein Precursors / immunology*
  • Prothrombin / immunology*
  • Sensitivity and Specificity
  • Vitamin K Deficiency / diagnosis

Substances

  • Antibodies, Monoclonal
  • Biomarkers
  • Protein Precursors
  • acarboxyprothrombin
  • Prothrombin
  • Calcium