[32] High-performance liquid chromatography-based thiorbarbituric acid tests

doi:10.1016/S0076-6879(94)33035-2

Methods in Enzymology

Volume 233, 1994, Pages 314-318

https://doi.org/10.1016/S0076-6879(94)33035-2 Get rights and content

Publisher Summary

The thiobarbituric acid (TBA) test is one of the oldest and most frequently used tests for measuring the peroxidation of fatty acids, membranes, and food products. In the food and dairy industries, the test is used as a general measure of lipid rancidity, while in medicine it remains the most commonly applied assay for lipid peroxidation to provide evidence of the involvement of free radicals in disease pathologies. The test is popular because it is simple and inexpensive. The sample under test is heated with TBA at low pH, and a pink chromogen is measured for absorbance at, or close to, 532 nm, or its fluorescence at 553 nm. The TBA test is often said to measure malondialdehyde (MDA) formed in peroxidizing lipid systems. Calibration of the test is achieved using MDA prepared in the laboratory by hydrolysis of 1,1,3,3-tetramethoxypropane or 1,1,3,3-tetraethoxypropane, and so results are frequently expressed as micromolar MDA equivalents. The availability of the technique of high-performance liquid chromatography (HPLC) is instrumental in the reevaluation of the TBA test. Many of the HPLC-based assays for the TBA test available at present have made a considerable contribution to the understanding of what the TBA test actually measures.

References (20)

H. Kosugi et al.
Anal. Biochem.
(1987)
H. Esterbauer et al.
Free Radical Biol. Med.
(1991)
J.M.C. Gutteridge et al.
Biochem. Med.
(1978)
C.R. Wade et al.
Life Sci.
(1988)
R.P. Bird et al.
Anal. Biochem.
(1983)
H.S. Gilbert et al.
Anal. Biochem.
(1984)
J.M.C. Gutteridge
FEBS Lett.
(1981)
B. Halliwell et al.
FEBS Lett.
(1981)
H.I. Kohn et al.
J. Pharmacol. Exp. Ther.
(1944)
V. Nair et al.
Lipids
(1984)

There are more references available in the full text version of this article.

Cited by (161)

Effect of Occupational Exposure to Herbicides on Oxidative Stress in Sprayers
2021, Safety and Health at Work
Herbicides such as glyphosate, paraquat, and 2,4-dichlorophenoxyacetic acid have been reported to cause adverse side effects through production of reactive oxygen species. However, there were no data representing the adverse effects of a mixture herbicide usage in farmers, especially the changes in oxidative marker and antioxidant defense. This study aimed to determine the urinary malondialdehyde (MDA) and glutathione (GSH) level in farmers using mixed herbicides.
Ninety-three farmers were recruited, and two spot urine samples (before and after work) were collected. The urinary MDA level was evaluated by thiobarbituric acid reactive substance assay, and the urinary GSH level was determined using the enzymatic recycling method.
Sixty-two percent of the participants were men, and 59% of the participants worked in a farm for 20–40 years. The common combinations of herbicide usage were glyphosate with 2,4-dichlorophenoxyacetic acid (36.5%). There was no significant difference between pre- and post-work urinary MDA and GSH levels among the 3 groups of herbicides. However, the urinary MDA levels in farmers using the combination of glyphosate and paraquat were significantly higher than those found in farmers using glyphosate alone. The associated factors with changes in MDA levels found that the exposure intensity index (B = 0.154), the cumulative exposure intensity index (B = 0.023), and wearing gloves while working (B = −2.347) were found to be significantly associated with MDA level.
The results suggest that the combined use of glyphosate and paraquat caused a significant increase in urinary MDA levels. Moreover, intensity of exposure to herbicide and wearing gloves were associated with the level of MDA.
The diverse effects of α- And γ -tocopherol on chicken liver transcriptome
2017, Poultry Science
Citation Excerpt :
Analytical results are excluded from the data presentation, as there were no differences among vitamin E suplemented groups. The methodology of Wong et al. (1987), modified by Chirico (1994) and Fukunaga et al. (1995), was implemented to determine the concentrations of malondialdehyde (MDA) in blood plasma using HPLC. In order to determine the MDA concentrations in liver, a modified protocol of the methodology of Vilà et al. (2002) was used.
α-Tocopherol is the form of vitamin E with the highest biological value and is almost exclusively considered as vitamin E in feed and feed supplements. Because γ-tocopherol, the predominant form of vitamin E naturally present in chicken feed, is not considered as a source of vitamin E, its re-evaluation with newer methods might be important.
Despite γ-tocopherol's lower estimated biological value, it has been shown to be effective in reducing reactive nitrogen species, regulating immune and inflammatory processes, and diminishing the risk of metabolic perturbations and associated diseases. A 30-day nutritional trial in broiler chickens (Ross 308) was conducted to investigate how specific forms of vitamin E (α- and γ-tocopherol) and their combination impact liver gene expression when oxidative susceptibility of the organism is induced by high n-3 polyunsaturated fatty acids (PUFA) intake (linseed oil). Thirty-six one-day-old male broilers were fed a diet enriched with 5% linseed oil. A control group (Cont; N = 10) was used as a reference group, Tα (N = 10) was supplemented with 67 mg/kg RRR-α-tocopherol, Tγ (N = 8) with 67 mg/kg RRR-γ-tocopherol, and Tαγ (N = 8) with a combination of 33.5 mg/kg of each tocopherol. Beside oxidative stress indicators, whole chicken genome microarray analysis was performed on liver RNA and selected differentially expressed genes were confirmed by real time quantitative PCR. α-Tocopherol alone and in combination with γ-tocopherol was able to prevent lipid oxidation, which was also supported by transcriptome analysis. The effect of γ-tocopherol was evident in the expression of genes involved in inflammatory processes and immune response, while α-tocopherol affected genes involved in lipid and cholesterol metabolism. Both isomers of vitamin E influenced the transcription of genes, which are related to improved fat oxidation and enhanced glucose sparing.
Gastroprotective activity of the rhizome ethanol extract of Zingiber simaoense Y. Y. Qian in rats
2016, Journal of Ethnopharmacology
Citation Excerpt :
The heparinized blood was centrifuged at 1831 g for 10 min and the plasma obtained was kept at −20 °C until analysis. The MDA content was measured following the procedure described by Chirico (1994) with slight modification. Plasma samples (0.5 mL) were mixed with 1.1 mL of 10% trichloroacetic acid containing 50 ppm butylated hydroxytoluene.
Zingiber simaoense Y. Y. Qian belongs to the Zingiberaceae family. Its rhizome has been used in Thai folk medicine to relieve gastric disorders; however, scientific evidence of its pharmacological activities has not yet been revealed.
This study was designed to validate the gastroprotective activity and to identify possible mechanisms of gastroprotection of Z. simaoense rhizome ethanol extract (ZSE) in rats.
The gastroprotective effect of ZSE was tested using models of gastric ulcers induced by acidified ethanol, indomethacin, and restraint water immersion stress. Models for determination of gastric wall mucus secretion and plasma malondialdehyde levels as well as pylorus ligation were used to explore the mechanisms of action.
After oral administration by intragastric gavage, ZSE 7.5, 15, and 30 mg/kg or cimetidine 100 mg/kg significantly inhibited the formation of gastric ulcer in all gastric ulcer models. The gastric wall mucus amount was significantly higher than that of the ulcer control group, plasma malondialdehyde levels were normalized, and gastric secretion was partly inhibited by pretreatment with ZSE.
This study demonstrates the gastroprotective activity of ZSE in rats. The mechanisms of action of ZSE may depend on its ability to maintain the integrity of gastric wall mucus through the protection of gastric mucus, and/or by increasing the gastric mucus synthesis and secretion through prostaglandin synthesis. Moreover, the antioxidant activity of ZSE may also contribute to its mechanism of gastroprotection.
Prealbumin improves death risk prediction of BNP-added Seattle Heart Failure Model: Results from a pilot study in elderly chronic heart failure patients
2013, International Journal of Cardiology
An accurate prognosis prediction represents a key element in chronic heart failure (CHF) management. Seattle Heart Failure Model (SHFM) prognostic power, a validated risk score for predicting mortality in CHF, is improved by adding B-type natriuretic peptide (BNP). We evaluated in a prospective study the incremental value of several biomarkers, linked to different biological domains, on death risk prediction of BNP-added SHFM.
Troponin I (cTnI), norepinephrine, plasma renin activity, aldosterone, high sensitivity-C reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), interleukin 2 soluble receptor, leptin, prealbumin, free malondialdehyde, and 15-F_2t-isoprostane were measured in plasma from 142 consecutive ambulatory, non-diabetic stable CHF (mean NYHA-class 2.6) patients (mean age 75 ± 8 years). Calibration, discrimination, and risk reclassification of BNP-added SHFM were evaluated after individual biomarker addition.
Individual addition of biomarkers to BNP-added SHFM did not improve death prediction, except for prealbumin (HR 0.49 CI: (0.31–0.76) p = 0.002) and cTnI (HR 2.03 CI: (1.20–3.45) p = 0.009). In fact, with respect to BNP-added SHFM (Harrell's C-statistic 0.702), prealbumin emerged as a stronger predictor of death showing the highest improvement in model discrimination (+ 0.021, p = 0.033) and only a trend was observed for cTn I (+ 0.023, p = 0.063). These biomarkers showed also the best reclassification statistic (Integrated Discrimination Improvement—IDI) at 1-year (IDI: cTnI, p = 0.002; prealbumin, p = 0.020), 2-years (IDI: cTnI, p = 0.018; prealbumin: p = 0.006) and 3-years of follow-up (IDI: cTnI p = 0.024; prealbumin: p = 0.012).
Individual addition of prealbumin allows a more accurate prediction of mortality of BNP enriched SHFM in ambulatory elderly CHF suggesting its potential use in identifying those at high-risk that need nutritional surveillance.
Sweet-type star fruit supplementation controls oxidative stress status and enhances the community walking capacity among elderly Thai
2023, BMC Complementary Medicine and Therapies
Supplementation of Sweet-type Star Fruit Product Controls the Oxidative Stress Status and Enhances the Walking Capacity from Home-Walking Exercise Among Dwelling Elderly in Chiang Mai Province
2023, Research Square

View all citing articles on Scopus

View full text

[32] High-performance liquid chromatography-based thiorbarbituric acid tests

Publisher Summary

Anal. Biochem.

Free Radical Biol. Med.

Biochem. Med.

Life Sci.

Anal. Biochem.

Anal. Biochem.

FEBS Lett.

FEBS Lett.

J. Pharmacol. Exp. Ther.

Lipids