Original ArticlesIsolation of Ureaplasma urealyticum From the Amniotic Cavity and Adverse Outcome in Preterm Labor☆
Section snippets
Materials and methods
The study population consisted of patients admitted with diagnosis of preterm labor with intact membranes and singleton gestation who underwent amniocentesis between January 1993 and October 1996 for assessment of the microbiologic status of the amniotic cavity. The study was carried out at Seoul National University Hospital of Seoul, Korea. All patients admitted with the diagnosis of preterm labor and intact membranes were offered amniocentesis for microbiologic assessment of the amniotic
Results
One hundred eighty-one patients were included in this study. The prevalence of positive AF culture was 11.6% (21 of 181). Microorganisms isolated from the amniotic cavity included U urealyticum (n = 13), Staphylococcus aureus (n = 2), Enterococcus sp. (n = 2), Lactobacillus sp. (n = 2), Acinetobacter sp. (n = 2), and one isolate each of Candida albicans, coagulase-negative Staphylococcus and Porphyromonas asaccharolytica. The coagulase-negative Staphylococcus was isolated from a woman with a
Discussion
This study was conducted to determine the clinical significance of microbial invasion of the amniotic cavity with U urealyticum. The relevance of this issue is underscored by data demonstrating that U urealyticum is the most frequent microbial isolate from AF in patients with preterm labor,2., 3., 4., 5., 9., 12. preterm premature rupture of membranes (PROM),6., 7. term PROM,23 and spontaneous labor at term.24 Yet, in many institutions, mycoplasma cultures are not part of the standard
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2023, Cell ReportsOptimization and validation of two multiplex qPCR assays for the rapid detection of microorganisms commonly invading the amniotic cavity
2022, Journal of Reproductive ImmunologyCitation Excerpt :In women with clinical chorioamnionitis (Romero et al., 2015b) or histological chorioamnionitis (Park et al., 2017), the prevalence of a positive amniotic fluid culture is 46 % and 53 %, respectively. Overall, based on cultivation results, MIAC occurs in approximately 25 % of women who deliver preterm (Bobitt and Ledger, 1978; Miller et al., 1980; Bobitt et al., 1981; Wallace and Herrick, 1981; Hameed et al., 1984; Wahbeh et al., 1984; Gravett et al., 1986; Leigh and Garite, 1986; Romero et al., 1988a, b; Romero et al., 1988c, d; Romero et al., 1989; Skoll et al., 1989; Romero et al., 1990a, b; Gauthier et al., 1991; Romero et al., 1991; Coultrip and Grossman, 1992; Watts et al., 1992; Andrews et al., 1995; Yoon et al., 1996; Elimian et al., 1998; Gomez et al., 1998; Hussey et al., 1998; Oyarzun et al., 1998; Rizzo et al., 1998; Yoon et al., 1998; Gonzalez-Bosquet et al., 1999; Locksmith et al., 1999; Ovalle et al., 2000; Yoon et al., 2000, 2001; Jacobsson et al., 2003; Romero and Lockwood, 2009). However, given the challenges of identifying MIAC through culture (DiGiulio et al., 2008; Han et al., 2009), this may be an underestimation.
Antibiotic administration can eradicate intra-amniotic infection or intra-amniotic inflammation in a subset of patients with preterm labor and intact membranes
2019, American Journal of Obstetrics and GynecologyCitation Excerpt :Therefore, in practice, clinicians rely on signs and symptoms of clinical chorioamnionitis (eg, fever, maternal tachycardia, etc) to exclude intra-amniotic infection. However, it is now well established that these clinical signs are both insensitive and nonspecific for the identification of intra-amniotic infection in both preterm6,33,121,125,171 and term136,137,172–176 gestations. This is also the case for maternal circulating white blood cell count and other biomarkers of the acute phase response (such as serum C-reactive protein).18,121,167,177,178
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Supported by grant No. 97-04-03-08-01-3 from the Korea Science and Engineering Foundation (KOSEF).
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Dr. Romero has participated as a private citizen, not as an agent of the US government or of any of the universities to which he holds appointments.