Elsevier

The Journal of Pediatrics

Volume 132, Issue 2, February 1998, Pages 349-351
The Journal of Pediatrics

Eight-epi-PGF: A possible marker of lipid peroxidation in term infants with severe pulmonary disease,☆☆,,★★

https://doi.org/10.1016/S0022-3476(98)70459-7Get rights and content

Abstract

A prostaglandin F2-like compound, 8-epi-PGF, formed from oxidation of arachidonate, has been proposed as an indicator of lipid peroxidation. We determined whether tracheal aspirate or urinary 8-epi-PGF levels would differ over time or between infants in a control group and infants with severe respiratory failure. We correlated tracheal aspirate 8-epi-PGF levels with the fraction of inspired oxygen and with mean airway pressures at 24 and 48 hours of life. Levels in tracheal aspirates were in the range of 0 to 36 pg/μg of fSC of IgA and were higher in infants with severe pulmonary disorders compared with those in infants in the control group (p < 0.02). Urinary concentrations did not discriminate between sick infants and infants in the control group. (J Pediatr 1998;132:349-51)

Section snippets

Patient Population

The patient populations included a total of 36 term infants grouped as follows. Group A: 15 term healthy infants, gestational age 39 ± 1 week, born by vaginal delivery. Urine samples were collected in each infant as a single urination during the periods at 0 to 6 hours, 6 to 12 hours, 12 to 24 hours, and 24 to 48 hours after birth. Group B: 15 term infants, gestational age 38 ± 1 week, who had respiratory failure requiring assisted ventilation because of respiratory distress syndrome, meconium

RESULTS

The urine 8-epi-PGF levels in group A at 0 to 6, 6 to 12, 12 to 24, and 24 to 48 hours of life were 5074 ± 1904, 6523 ± 3196, 5512 ± 2572, and 6011 ± 2610 pg/mg creatinine, respectively (p > 0.1). The 8-epi-PGF levels in the urine of group B at 24 and 48 hours were 5440 ± 4358 and 3609 ± 1933 pg/mg creatinine. These values were not significantly different (p > 0.2) from those of the healthy term infants.

Tracheal aspirate 8-epi-PGF levels in the infants receiving ventilatory assistance

DISCUSSION

Oxidative free radical lipid peroxidation of cell membranes has been difficult to quantitate in vivo because of inadequacy of detection methods. The production of F2 isoprostanes is markedly increased in animal models of oxidant injury1 and in plasma of human cigarette smokers.8 Thus quantification of these products of lipid peroxidation may represent an important advance in the assessment of oxidant injury in vivo.

Exposure of the human neonatal lung to FIO2 = 1.0 would appear to constitute a

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From the Sections of Neonatal Medicine and of Allergy and Immunology, The Children's Mercy Hospital, Department of Pediatrics, The University of Missouri-Kansas City School of Medicine, Kansas City, Missouri.

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Supported in part by a grant from the Research and Education Fund of The Children's Mercy Hospital (S. Goil), from Wyeth Pediatrics Neonatal Research Fund Award (S. Goil), and a Children's Mercy Hospital Physician Scientist Award (W. Truog).

Reprint requests: William E. Truog, MD, Section of Neonatal Medicine, The Children's Mercy Hospital, 2401 Gillham Rd., Kansas City, MO 64108-9898.

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