Eight-epi-PGF2α: A possible marker of lipid peroxidation in term infants with severe pulmonary disease☆,☆☆,★,★★
Section snippets
Patient Population
The patient populations included a total of 36 term infants grouped as follows. Group A: 15 term healthy infants, gestational age 39 ± 1 week, born by vaginal delivery. Urine samples were collected in each infant as a single urination during the periods at 0 to 6 hours, 6 to 12 hours, 12 to 24 hours, and 24 to 48 hours after birth. Group B: 15 term infants, gestational age 38 ± 1 week, who had respiratory failure requiring assisted ventilation because of respiratory distress syndrome, meconium
RESULTS
The urine 8-epi-PGF2α levels in group A at 0 to 6, 6 to 12, 12 to 24, and 24 to 48 hours of life were 5074 ± 1904, 6523 ± 3196, 5512 ± 2572, and 6011 ± 2610 pg/mg creatinine, respectively (p > 0.1). The 8-epi-PGF2α levels in the urine of group B at 24 and 48 hours were 5440 ± 4358 and 3609 ± 1933 pg/mg creatinine. These values were not significantly different (p > 0.2) from those of the healthy term infants.
Tracheal aspirate 8-epi-PGF2α levels in the infants receiving ventilatory assistance
DISCUSSION
Oxidative free radical lipid peroxidation of cell membranes has been difficult to quantitate in vivo because of inadequacy of detection methods. The production of F2 isoprostanes is markedly increased in animal models of oxidant injury1 and in plasma of human cigarette smokers.8 Thus quantification of these products of lipid peroxidation may represent an important advance in the assessment of oxidant injury in vivo.
Exposure of the human neonatal lung to FIO2 = 1.0 would appear to constitute a
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Cited by (0)
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From the Sections of Neonatal Medicine and of Allergy and Immunology, The Children's Mercy Hospital, Department of Pediatrics, The University of Missouri-Kansas City School of Medicine, Kansas City, Missouri.
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Supported in part by a grant from the Research and Education Fund of The Children's Mercy Hospital (S. Goil), from Wyeth Pediatrics Neonatal Research Fund Award (S. Goil), and a Children's Mercy Hospital Physician Scientist Award (W. Truog).
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Reprint requests: William E. Truog, MD, Section of Neonatal Medicine, The Children's Mercy Hospital, 2401 Gillham Rd., Kansas City, MO 64108-9898.
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