Original contributionMeasurement of n-Alkanals and hydroxyalkenals in biological samples☆
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Cited by (53)
Commercial luncheon meat products and their in vitro gastrointestinal digests contain more protein carbonyl compounds but less lipid oxidation products compared to fresh pork
2020, Food Research InternationalCitation Excerpt :Standard solutions (0 – 20 nmol/ml) were made with 1,1,3,3-tetramethoxypropane and results were expressed as nmol MDA/g (digested) meat. Levels of free reactive 4-HNE and hexanal were analyzed after formation of their fluorescent derivatives with 1,3-cyclohexanedione through HPLC (Supelcosil LC18 column, 25 cm × 4.6 mm × 5 µm) based on the method of Holley, Walker, Cheeseman & Slater (1993). Concentrations were determined by comparing the peak areas with those obtained from the standard curves of 4-HNE (0 – 2 nmol/mL) and hexanal (0 – 40 nmol/mL) and results were provided in nmol/g (digested) meat.
The potential of herbs and spices to reduce lipid oxidation during heating and gastrointestinal digestion of a beef product
2017, Food Research InternationalChemistry and analysis of HNE and other prominent carbonyl-containing lipid oxidation compounds
2017, Free Radical Biology and MedicineCitation Excerpt :The top-down approach was supplemented with typical nano LC-ESI-MS/MS analysis of peptides from a tryptic digest as described above to map the specific sites of modification [128]. The physiological serum concentrations of free aldehydes may depend on a number of factors, such as the rate of production versus breakdown or detoxification, as well as the amount of adduction of aldehydes to biomolecules [129]. Moreover, Schiffs base and even Michael adduct formation is reversible unless the adducts are stabilized by reduction or a rearrangement such as cyclization, which may affect quantification.
RP-HPLC-fluorescence analysis of aliphatic aldehydes: Application to aldehyde-generating enzymes HACL1 and SGPL1
2014, Journal of Lipid ResearchCitation Excerpt :Subsequently, we checked to which extent aldehydes with rather similar chain length could be discriminated: a good resolution of different long-chain aldehydes was obtained, and the elution time related to the number of carbon atoms (Fig. 4). The presence of proteins can affect the recovery of aliphatic aldehydes (e.g., Schiff base with amino groups, hydrophobic interactions, trapping in pelleted proteins or in interphase during extraction), and negative effects of proteins have been observed also in CHD-based assays containing no or less methanol [59% recovery for C6-al in presence of 16.7 mg rat liver/ml assay mixture (28); 65–72% recovery for C7-al in presence of 50 μl human plasma/ml assay mixture (30); chain length-dependent decrease of recovery, down to 8% for C10-al, in presence of 0.33 ml human plasma/ml assay mixture (29)]. Nonetheless, recovery of medium-chain aldehydes (C13-al) was excellent under our conditions [33% (v/v) methanol during derivatization] in presence of increasing amounts of tissue homogenate.
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This work was supported by grants from the Wellcome Trust and the Association for International Cancer Research.
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Deceased.