Abstract
Purpose
To investigate whether breast-milk composition and microbiota differ in healthy mothers and mothers with celiac disease (CD) to ultimately contribute to identify additional factors determining CD risk.
Methods
Breast-milk samples from healthy mothers (n = 12) and mothers with CD (n = 12) were collected. Cytokines and secretory immunoglobulin A (sIgA) were analyzed by bead-arrays and flow cytometry and human milk oligosaccharides (HMOs) were assessed by capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection. Breast-milk microbiota composition was analyzed by conventional and quantitative real-time PCR.
Result
Breast milk from CD mothers showed significantly lower levels of interleukin (IL) 12p70 (P < 0.042), transforming growth factor (TGF)-β1 (P < 0.018) and sIgA (P < 0.003) and almost significantly lower levels of interferon (IFN)-γ (P < 0.058). Six mothers in each group belonged to the secretor Le(a−b+) type, one to the secretor Le(a−b−) type and five to the non-secretor Le(a+b−) type. CD mothers of non-secretor Le(a+b−) type showed increased Lacto-N-tetraose content (P < 0.042) compared with healthy mothers. CD mothers’ milk showed reduced gene copy numbers of Bifidobacterium spp. (P < 0.026) and B. fragilis group (P < 0.044).
Conclusion
CD mothers’ breast milk is characterized by a reduced abundance of immunoprotective compounds (TGF-β1 and sIgA) and bifidobacteria. The reduction in these components could theoretically diminish the protective effects of breast-feeding on the child’s future risk of developing CD.
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Abbreviations
- ACN:
-
Acetonitrile
- APTS:
-
9-Aminopyrene-1,4,6-trisulfonate
- CD:
-
Celiac disease
- Ct:
-
Cycle threshold
- DGGE:
-
Denaturing gradient gel electrophoresis
- DFL:
-
Difucosyllactose
- DF LNH:
-
Difucosyllacto-N-hexaose
- CE-LIF:
-
Electrophoresis-laser-induced fluorescence
- ESPGHAN:
-
European Society of Pedriatric Gastroenterology Hepatology and Nutrition
- Fuc:
-
Fucose
- F LNH:
-
Fucosyllacto-N-hexaose
- FL:
-
Fucosyllactose
- FUT:
-
Fucosyltransferase
- Gal:
-
Galactose
- Glu:
-
Glucose
- GFD:
-
Gluten free diet
- TGF:
-
Transforming growth factor
- HLA:
-
Human leukocyte antigen
- HMO:
-
Human milk oligosaccharide
- IFN:
-
Interferon
- IL:
-
Interleukin
- N-Acetyl:
-
N-Acetylglucosamine
- LNT:
-
Lacto-N-tetraose
- LIF:
-
Laser-induced fluorescence
- LNFP:
-
Lacto-N-fucopentose
- LNDFH:
-
Lacto-N-difucosylhexaose
- Le:
-
Lewis
- Se:
-
Secretor
- sIgA:
-
Secretory immunoglobulin A
- Neu5Ac:
-
Sialic acid
- SL:
-
Sialyllactose
- TNF:
-
Tumor necrosis factor
- TFA:
-
Trifluoroacetic acid
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Acknowledgments
This work was supported by Grants AGL2011-25169 and Consolider Fun-C-Food CSD2007-00063 from the Spanish Ministry of Economy and Competitiveness (MINECO). The scholarship to M. Olivares from Consejo Superior de Investigaciones Científicas (CSIC) is fully acknowledged.
Conflict of interest
The authors declare that they do not have conflict of interest.
Ethical standards
This study was conducted according to the guidelines laid down in the Declaration of Helsinki and all procedures involving human subjects/patients were approved by the Committee on Ethical Practice at the Hospital Universitari Sant Joan (Tarragona, Spain). All the mothers participating in the study gave written informed consent.
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Olivares, M., Albrecht, S., De Palma, G. et al. Human milk composition differs in healthy mothers and mothers with celiac disease. Eur J Nutr 54, 119–128 (2015). https://doi.org/10.1007/s00394-014-0692-1
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DOI: https://doi.org/10.1007/s00394-014-0692-1