Abstract
The determination of lymphocyte subsets utilizing monoclonal antibodies and flow cytometry has become essential in the evaluation of immunological status. Using a standardized method it was found that in healthy children the percentage of CD 8+ (Leu 2+) positive cells increases significantly (P<0.01) during infancy, whereas the percentage of CD 4+ (Leu 3+) positive cells decreases with age (P<0.01). The percentage of CD 3+ (Leu 4+) cells remains constant. The ratio of CD 4/CD 8 positive cells is significantly (P<0.001) higher in infants than in older children. Other subpopulations (HLA DR+, Leu 7) were found to be constant in all age groups. For the comparison of data on lymphocyte subsets obtained by flow cytometry a standardized test procedure is important.
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Abbreviations
- PBML:
-
peripheral blood mononuclear leucocytes
- PBS-azide:
-
phosphate buffered saline azid 0.1% NaN3, ph 7.2
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Remy, N., Oberreit, M., Thoenes, G. et al. Lymphocyte subsets in whole blood and isolated mononuclear leucocytes of healthy infants and children. Eur J Pediatr 150, 230–233 (1991). https://doi.org/10.1007/BF01955518
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DOI: https://doi.org/10.1007/BF01955518