Neutralising SARS-CoV-2 antibodies of human milk in included studies
| Author | Neutralisation assay | Number of individuals | Neutralising threshold | Pasteurisation methods | SARS-CoV-2 neutralisation | Correlation of neutralisation abilities | Ratio of neutralisation IC50 values of serum: human milk | Ratio of neutralisation IC50 values of serum: human milk after Holder pasteurisation |
| Van Keulen et at 25 | SARS CoV-2 clinical isolate on Vero E6 cell lined-based pseudovirus | 29 | 50% as compared with control | Holder and high-pressure | 26%; 8 individuals | Higher neutralisation titres correlated with higher concentration of IgA | NA | NA |
| Favara et al 16 | SARS-CoV-2 S-antigen-expressing pseudovirus | 1 | 50% as compared with control | Holder | Yes | NA | 1:1 | Neutralisation decreased to 20% from pre-Holder pasteurisation |
| Pace et al 22 | Microneutralisation assays (using Vero E6/TMPRSS2 cells) | 18 | 50% as compared with control | Not done | 61%;11 individuals | Higher neutralisation titre were correlated with higher concentration of RBD-reactive IgA | NA | NA |
NA, not tested; RBD, receptor binding domain; S-protein, spike protein.