Primer sequences for quantitative real-time PCR
| Gene | Species | Accession number | Primer sequence | Amplicon length, nt |
| 18s | Rat | NR_046237.1 | 5′-GTAACCCGTTGAACCCCATT-3′ 5′-CCATCCAATCGGTAGTAGCG-3′ | 151 |
| iNOS | Ovine | XM_004012488.3 | 5′-CCTGGCAACGGAGAGAAACT-3′ 5′-CGTGAGCAAAAGCACAGAACT-3′ | 124 |
| Claudin-1 | Ovine | NM_001185016.1 | 5′-GACGACGAGGCACAGAAGAT-3′ 5′-CAAAACAGCCAGACCTGAAA-3′ | 75 |
| Claudin-5 | Ovine | XM_015105318.1 | 5′-CCTGGACCACAACATCGTGA-3′ 5′-AGCACCGAGTCGTACACTTT-3′ | 108 |
| Occludin | Bovine | NM_001082433.1 | 5′-GCAGGAAGTGCCTTTGGTAG-3′ 5′-CCATAGCCATAACCGTAGCC-3′ | 68 |
iNOS, inducible nitric oxide synthase.