Relative abundance of α1, α2, α3, β1and β2 subunit isoforms and the ratio of (α1+α2)/(β1+β2) in purified erythrocyte membranes in groups 1, 2, and 3
|
Group 1 (preterm neonates, 28–32 weeks) |
Group 2 (preterm neonates, 33–36 weeks) |
Group 3 (term neonates, 37–42 weeks) | |
| α1 | 176 (11)1-150 | 132 (23)1-151 | 100 (18) |
| α2 | 100 (10) | 108 (16) | 100 (27) |
| α3 | 0 | 0 | 0 |
| α1+α2 | 152 (10)1-150 | 122 (20)1-151 | 100 (37) |
| α1/α2 | 163 (10)1-150 | 113 (16) | 100 (11) |
| β1 | 94 (8) | 93 (7) | 100 (19) |
| β2 | 51 (16)1-150 | 87 (32) | 100 (12) |
| β1+β2 | 80 (8)1-150 | 101 (12) | 100 (14) |
| β1/β2 | 188 (17)1-150 | 125 (23) | 100 (21) |
| α1+α2/β1+β2 | 189 (16)1-150 | 124 (31) | 100 (20) |
Purified erythrocyte membranes were obtained from pooled blood samples within each group, and western blot analysis (n = 6) was performed using subunit isoform specific antibodies. See the text for details. Results are means (SD) and are expressed as percentage of the findings in term neonates (group 3). The presence of α3subunit isoforms could not be documented.
↵1-150 p < 0.05 v groups 2 and 3 (one factor analysis of variance; Fisher protected least squares difference test);
↵1-151 p < 0.05 v group 3 (one factor analysis of variance; Fisher protected least squares difference test).