RT Journal Article
SR Electronic
T1 PC.26 Feasibility of Magnetic Resonance Spectroscopy in Examining Thalamic Metabolite Concentrations in a Multi-Centre Study of Neonatal Encephalopathy
JF Archives of Disease in Childhood - Fetal and Neonatal Edition
JO Arch Dis Child Fetal Neonatal Ed
FD BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health
SP A44
OP A45
DO 10.1136/archdischild-2014-306576.128
VO 99
IS Suppl 1
A1 PJ Lally
A1 DL Price
A1 A Bainbridge
A1 SS Pauliah
A1 P Satodia
A1 SC Wayte
A1 L Abernethy
A1 M Turner
A1 AN Basheer
A1 A Alavi
A1 O Kirmi
A1 B Jones
A1 S Shankaran
A1 EB Cady
A1 S Thayyil
YR 2014
UL http://fn.bmj.com/content/99/Suppl_1/A44.3.abstract
AB Background Proton magnetic resonance spectroscopy (MRS) has high prognostic value in hypoxic ischaemic encephalopathy (HIE), however its multi-centre application is limited by inconsistencies between scanners and protocols. N-acetylaspartate (NAA) is predominantly neuronal: cerebral NAA concentration may be a more reliable HIE-severity biomarker than lactate/NAA. Objective To quantify the inter-site and inter-subject variability of NAA concentration measurements. Design/Methods We recruited 5 healthy adult volunteers (aged 24–38, 2 male, 3 female) whom we scanned at 3 UK sites participating in a multi-centre neonatal-brain study (University Hospital, Coventry (UHC); Alder Hey Children’s Hospital, Liverpool (AH); University College Hospital, London (UCLH)). Thalamic NAA concentration was measured using the neonatal brain-water concentration reference protocol (15 × 15 × 15mm3 voxel; PRESS; water-suppressed TR/TE = 2s/288&60ms; TR/TE = 5s/60ms; non-water-suppressed TR = 10s, TE = 60/124/205/316/495/1000 ms). Spectra were post-processed in jMRUI and metabolites fitted with LCModel. Results One volunteer was unavailable for scanning at AH. The mean (SD) NAA concentrations across the remaining four subjects were 15.5(3.4)mmol/kg wet weight, 14.4(0.1) mmol/kg wet weight, and 15.2(0.1) mmol/kg wet weight at UHC, AH and UCLH respectively. This corresponds to a maximum inter-site group mean variation (range/mean) of 8%. The inter-subject variability of mean NAA concentration (SD/mean) was 10%. Conclusions The variation of thalamic NAA concentration between sites was 8% and the standard deviation across subjects was 10%, hence [NAA] may be suitable for multi-centre studies. Abstract PC.26 Figure