TY - JOUR T1 - PM.11 A Label-Free SRM Workflow Identifies a Subset of Pregnancy Specific Glycoproteins as Novel Predictive Markers of Early-Onset Pre-Eclampsia JF - Archives of Disease in Childhood - Fetal and Neonatal Edition JO - Arch Dis Child Fetal Neonatal Ed SP - A28 LP - A28 DO - 10.1136/archdischild-2013-303966.096 VL - 98 IS - Suppl 1 AU - RT Blankley AU - C Fisher AU - M Westwood AU - RA North AU - Baker Philip AU - M Walker AU - T Whetton AU - L McCowan AU - G Dekker AU - R Unwin AU - JE Myers Y1 - 2013/04/01 UR - http://fn.bmj.com/content/98/Suppl_1/A28.3.abstract N2 - Objective The aim of this study was to identify and verify plasma protein markers which may add to predictive algorithms for pre-eclampsia (PE) in asymptomatic nulliparous women. Methods We used a quantitative mass spectrometry (MS) approach to identify proteins with abundance changes in plasma (15 weeks) taken from women who subsequently develop PE recruited to the international SCOPE study. We developed a novel, targeted, label-free MS method, selective reaction monitoring (SRM) which enabled robust and reproducible verification of these proteins in a further 100 samples (16 early-onset PE, 42 late-onset PE, 42 controls). Results We identified and quantified >500 plasma proteins, and prioritised a set of candidate predictive markers. The two most promising, Platelet Basic Protein (PBP/NAP-2) and Pregnancy-specific glycoprotein (PSG)-9 were selected for further verification. The SRM method was validated extensively using dilution experiments for PSG proteins and by comparison to a commercial ELISA for NAP-2. NAP-2 was only elevated in a subset of women with PE, however, peptides unique to PSG-9 and PSG-5 were consistently elevated in women with subsequent early onset PE (p < 0.01; AUCs 0.72–0.75). Other PSG peptides were not different between groups. Conclusion This study has identified specific PSG proteins as being predictive of early-onset PE. Importantly, use of a highly specific MS method has enabled measurement of individual PSG family members which has not been possible using antibody-based techniques. Future work is needed to determine whether these proteins will improve current prediction algorithms for the identification of PE in low risk nulliparous women. ER -