PT - JOURNAL ARTICLE AU - Härtel, C AU - Osthues, I AU - Rupp, J AU - Haase, B AU - Röder, K AU - Göpel, W AU - Herting, E AU - Schultz, C TI - Characterisation of the host inflammatory response to <em>Staphylococcus epidermidis</em> in neonatal whole blood AID - 10.1136/adc.2007.124685 DP - 2008 Mar 01 TA - Archives of Disease in Childhood - Fetal and Neonatal Edition PG - F140--F145 VI - 93 IP - 2 4099 - http://fn.bmj.com/content/93/2/F140.short 4100 - http://fn.bmj.com/content/93/2/F140.full SO - Arch Dis Child Fetal Neonatal Ed2008 Mar 01; 93 AB - Background: Coagulase-negative staphylococci (CoNS) are the most prevalent pathogens causing late-onset sepsis, and gestational age is the most important risk factor for these infections.Objective: To characterise innate immune responses to S epidermidis by assessment of whole blood in vitro cytokine production in a large group of preterm and term infants.Results: The S epidermidis-induced in vitro production of proinflammatory cytokines such as intracytoplasmic interleukin (IL) 6 and tumour necrosis factor α in cord blood samples was found to be dependent on gestational age (R = 0.279, 95% CI 0.10 to 0.44, p = 0.002; R = 0.251, 95% CI 0.07 to 0.41, p = 0.005, respectively; n = 121). In contrast, the production of anti-inflammatory cytokines such as IL10 and transforming growth factor β was not associated with gestational age. When different stimulation strategies were compared, a strong correlation was noted for cytokine responses after lipopolysaccharide and S epidermidis exposure—that is, IL6 (R = 0.431, 95% CI 0.29 to 0.55, p&lt;0.001, n = 161) and IL10 (R = 0.332, 95% CI 0.18 to 0.47, p&lt;0.001, n = 161). In addition, a lower IL6 production was found in supernatants of whole blood cultures infected with a clinically isolated IcaABD-positive (biofilm production) strain compared with a control IcaABD-negative ATCC strain (p = 0.009).Conclusions: These in vitro data suggest that proinflammatory responses to S epidermidis are dependent on gestational age in preterm infants, whereas the counteracting anti-inflammatory response to S epidermidis may not be directly related to gestational age. Individual host factors may have a role as well as bacterial determinants, such as biofilm production. Further studies are encouraged to investigate the different aspects of innate immune responses to CoNS in vivo.