RT Journal Article
SR Electronic
T1 Type I collagenases in bronchoalveolar lavage fluid from preterm babies at risk of developing chronic lung disease
JF Archives of Disease in Childhood - Fetal and Neonatal Edition
JO Arch Dis Child Fetal Neonatal Ed
FD BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health
SP F168
OP F171
DO 10.1136/fn.84.3.F168
VO 84
IS 3
A1 D G Sweet
A1 K J McMahon
A1 A E Curley
A1 C M O'Connor
A1 H L Halliday
YR 2001
UL http://fn.bmj.com/content/84/3/F168.abstract
AB OBJECTIVE To assess whether increased collagenolysis precedes severe chronic lung disease (CLD).METHODS Matrix metalloproteinase-1 (MMP-1) and MMP-8 (enzymes that degrade type I collagen, the main structural protein of lung extracellular matrix) were measured by enzyme linked immunosorbent assay in 100 bronchoalveolar lavage samples taken during the first 6 postnatal days from 45 ventilated preterm babies < 33 weeks gestation. The median value for each baby was calculated. CLD was defined as an oxygen requirement after the 36th week after conception.RESULTS MMP-8 levels in bronchoalveolar lavage fluid were higher (median 13 ng/ml) in 20 babies who developed CLD than in 25 without CLD (median 2 ng/ml). No MMP-1 was detected in any sample.CONCLUSIONS MMP-8 can be detected in bronchoalveolar lavage fluid from preterm babies, and higher levels are found in those who later develop CLD. MMP-8 may contribute to lung injury that occurs as a prelude to CLD.