Article Text

Download PDFPDF
8.8 Neonatal Encephalopathy in the Cooling Therapy era – Preliminary Cerebral Magnetic Resonance results from the Marble Consortium
  1. SS Pauliah1,
  2. PJ Lally1,
  3. A Bainbridge2,
  4. DL Price2,
  5. S Addison1,
  6. FM Cowan1,
  7. P Satodia3,
  8. SC Wayte3,
  9. A Soe4,
  10. S Pattnayak4,
  11. S Harigopal5,
  12. LJ Abernethy6,
  13. MA Turner6,
  14. P Clarke7,
  15. J Cheong8,
  16. SN Basheer1,
  17. A Alavi1,
  18. S Shankaran9,
  19. EB Cady2,
  20. S Thayyil1
  1. 1Imperial College, London, UK
  2. 2University College Hospital, London, UK
  3. 3University Hospitals Coventry and Warwickshire NHS Trusts, Coventry, UK
  4. 4Medway Maritime Hospital, Gillingam, UK
  5. 5Royal Victoria Informary, Newcastle, UK
  6. 6Alderhey Children’s Hospital, Liverpool, UK
  7. 7Norfolk and Norwich University Hospital, Norwich, UK
  8. 8The Royal Women’s Hospital, Melbourne, Victoria, Australia
  9. 9Wayne State University, Detroit, Michigan, USA


Background Although cerebral metabolic changes during neonatal encephalopathy (NE) have been well characterised using magnetic resonance spectroscopy (MRS) in single-centre studies, the widespread effect of therapeutic hypothermia is less clear.

Objective To describe patterns of brain injury in a prospective multi-centre cohort of infants with NE who had rescue hypothermic neuroprotection.

Design After MRS harmonisation on 3T MR scanners (Phillips, GE, Siemens), at six participating sites we performed magnetic resonance imaging (MRI) and MRS (single-voxel PRESS, 15 x 15 × x 15 mm3 thalamic voxel, TR = 2s/TE = 288 ms) on infants <2 wks who had whole-body cooling for NE at any of the MARBLE centres. We used jMRUI to process MRS data and calculate metabolite peak-area ratios.

Results Forty-three infants were recruited; 11(26%) had mild, 26(60%) moderate and 6(14%) had severe NE (<6h Sarnat staging). Moderate/severe basal ganglia and cortex were detected by MRI in 4(9%) and 1(2%) cases respectively, and 15(34%) had moderate/severe white matter injury. Thirteen (30%) had normal MRI.

Elevations in lactate (Lac)/N-acetylaspartate (NAA) were noted in 6(13%) cases, Lac/choline (Cho) in 5(11%) and Lac/ creatine (Cr) in 8(18%). MRS was normal in 28(65%) infants.

Abstract 8.8 Figure 1

Figure 1 displays metabolite peak-area ratios across clinical NE stages. Mean Lac ratios were higher and NAA ratios lower (p < 0.05) between severe and both mild and moderate NE, but not between mild and moderate groups

Conclusions In this predominantly moderate NE cohort, white matter injury was most common, and the majority had normal MRS metabolite ratios. Ongoing work seeks to establish whether these brain injury patterns are characteristic in the cooling therapy era.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.