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Accuracy of amplitude integrated EEG in a neonatal cohort
  1. E Evans1,
  2. S Koh3,
  3. JT Lerner2,
  4. R Sankar2,
  5. M Garg1
  1. 1Division of Neonatology & Developmental Biology and Neonatal Research Center Department of Pediatrics, David Geffen School of Medicine UCLA, Los Angeles, CA, USA
  2. 2Division of Pediatric Neurology, Department of Pediatrics, David Geffen School of Medicine UCLA, Los Angeles, CA, USA
  3. 3Division of Pediatric Neurology, University of Colorado School of Medicine, The Children's Hospital, Denver, CO, US
  1. Correspondence to Dr Meena Garg, Department of Pediatrics, Mattel Children's Hospital, David Geffen School of Medicine at UCLA, 10833 Le Conte Avenue, Room B2-375 MDCC, Los Angeles, CA 90095-1752, USA; mgarg{at}


Objective To determine the sensitivity and specificity of amplitude integrated electroencephalogram (aEEG) compared to simultaneous standard electroencephalogram (sEEG) for seizure detection and background discontinuity.

Design Prospective paired cohort.

Setting Tertiary academic neonatal intensive care unit.

Patients Infants were recruited from 2005 to 2008. Neonates requiring sEEG were recruited for simultaneous aEEG.

Interventions Following sEEG and aEEG, seizures were recorded as present or absent, and background was recorded as normal or discontinuous in each format.

Main outcome measures Presence of at least one seizure during recording. The background activity was reported as normal or discontinuous. Discontinuity of brain activity was further ranked as mild, moderate or severe.

Results 51 sEEG and aEEG studies were completed. 44 studies were analysed for presence of seizures and 46 were analysed for background discontinuity. Sensitivity for presence of seizures by aEEG was 80% and specificity was 50%. The proportion of infants with seizures were overdiagnosed by aEEG (63.6% vs 45.5% for sEEG p=0.045). Discontinuity of background activity had higher sensitivity (88.6%) and specificity (54.5%) when compared with seizure detection.

When stratified by indication for EEG, hypoxic episode (n=14) or suspected seizures (n=33), similar sensitivity for presence of seizure (80%) was noted by aEEG and sEEG. However the specificity of aEEG for seizure detection was higher in neonates undergoing EEG for suspected seizures (66.7% vs 22.2%).

Conclusions Background abnormalities were detected with fair accuracy by aEEG but aEEG criteria alone would result in the overdiagnosis of neonatal seizures. Therefore seizures noted on aEEG require sEEG confirmation prior to implementing anticonvulsant therapy for neonatal seizures.

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  • Conflicts of interest None.

  • Ethics approval This study was conducted with the approval of the UCLA Institutional Review Board: Office for Protection of Research Subjects (OPRS), 11000 Kinross Avenue, Suite 102, Box 951694, Los Angeles, California 90095-1694.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Patient consent Parental consent obtained.