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Cytomegalovirus remains viable in naturally infected breast milk despite being frozen for 10 days
  1. N Curtis1,2,3,
  2. L Chau2,
  3. S Garland4,5,
  4. S Tabrizi4,5,
  5. R Alexander6,
  6. C J Morley2,3,7
  1. 1Paediatric Infectious Diseases Unit, Department of General Medicine, Royal Children’s Hospital, Melbourne, Australia
  2. 2Department of Paediatrics, University of Melbourne, Royal Children’s Hospital, Melbourne, Australia
  3. 3Murdoch Children’s Research Institute, Melbourne, Australia
  4. 4Department of Clinical Microbiology and Infectious Diseases, Royal Women’s & Royal Children’s Hospitals, Melbourne, Australia
  5. 5Department of Obstetrics and Gynaecology, University of Melbourne, Royal Women’s Hospital, Melbourne, Australia
  6. 6Virology Laboratory, Department of Microbiology and Infectious Diseases, Royal Children’s Hospital, Melbourne, Australia
  7. 7Department of Neonatal Medicine, Royal Women’s and Royal Children’s Hospitals, Melbourne, Australia
  1. Correspondence to:
    Associate Professor Curtis
    Department of Paediatrics, University of Melbourne, Royal Children’s Hospital, Parkville, VIC 3052, Australia; nigel.curtisrch.org.au

Abstract

Cytomegalovirus culture positive breast milk was obtained from four mothers of very premature babies. The milk was stored at 0–5°C in a domestic refrigerator for 48 hours or frozen for different durations at −20°C. Cytomegalovirus survived in breast milk despite being frozen for 10 days at −20°C.

  • CMV, cytomegalovirus
  • RETCIF, rapid enhanced tissue culture immunofluorescence
  • breast milk
  • cytomegalovirus
  • freezing
  • infection

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Footnotes

  • Competing interests: none declared