Objective Physiological-based cord clamping (PBCC) led to a more stable cardiovascular adaptation and better oxygenation in preterm lambs, but in preterm infants, this approach has been challenging. Our aim was to assess the feasibility of PBCC, including patterns of oxygen saturation (SpO2) and heart rate (HR) during stabilisation in preterm infants using a new purpose-built resuscitation table.
Design Observational study.
Setting Tertiary referral centre, Leiden University Medical Centre, The Netherlands.
Patients Infants born below 35 weeks’ gestational age.
Interventions Infants were stabilised on a new purpose-built resuscitation table (Concord), provided with standard equipment needed for stabilisation. Cord clamping was performed when the infant was stable (HR >100 bpm, spontaneous breathing on continuous positive airway pressure with tidal volumes >4 mL/kg, SpO2 ≥25th percentile and fraction of inspired oxygen (FiO2) <0.4).
Results Thirty-seven preterm infants were included; mean (SD) gestational age of 30.9 (2.4) weeks, birth weight 1580 (519) g. PBCC was successful in 33 infants (89.2%) and resulted in median (IQR) cord clamping time of 4:23 (3:00–5:11) min after birth. There were no maternal or neonatal adverse events. In 26/37 infants, measurements were adequate for analysis. HR was 113 (81–143) and 144 (129–155) bpm at 1 min and 5 min after birth. SpO2 levels were 58%(49%–60%) and 91%(80%–96%)%), while median FiO2 given was 0.30 (0.30–0.31) and 0.31 (0.25–0.97), respectively.
Conclusion PBCC in preterm infants using the Concord is feasible. HR remained stable, and SpO2 quickly increased with low levels of oxygen supply.
Trial registration number NTR6095, results.
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What is already known on this topic?
Preterm infants requiring stabilisation or resuscitation are excluded from the beneficial effects of delayed cord clamping.
Clinical approaches in providing respiratory support prior to cord clamping still used a fixed time point for clamping.
Changes in heart rate and oxygen saturation of preterm infants before cord clamping are currently unknown.
What this study adds?
Physiological-based cord clamping using a new purpose-built resuscitation table is feasible and without adverse events for mother and infant.
Using the infant’s condition and transitional status as key determinant for the timing of cord clamping results in considerably later cord clamping than described previously.
Physiological-based cord clamping is associated with stable heart rate and improved oxygen saturation during transition in preterm infants.
Multiple trials in preterm infants have shown that delayed umbilical cord clamping (DCC), as compared with immediate umbilical cord clamping (ICC), reduces the risk of mortality and the need for blood transfusions.1 2 The mechanism of increased survival is not fully understood; however, the beneficial effect of DCC mainly was attributed to a larger circulating neonatal blood volume by increased placental transfusion.3 Preterm infants requiring stabilisation or resuscitation at birth were generally excluded from DCC and received ICC, because of the necessity to transfer these infants to a resuscitation table to provide respiratory support.
While it is recognised that the pulmonary and haemodynamic transitions at birth are intimately linked,4 5 current clinical practice recommends cord clamping at a fixed time point, irrespective of the infant’s transitional status. ICC can cause a sudden reduction in cardiac output, heart rate (HR) and blood pressure, whereas cord clamping after onset of ventilation (physiological-based cord clamping (PBCC)) avoids both this and the large disturbances in systemic and cerebral haemodynamics in experimental settings.5 6 These findings may explain the bradycardia and hypoxaemia that are often observed in preterm infants after cord clamping.7–9 Therefore, a more physiological approach to the timing of cord clamping is likely to be beneficial. Particularly preterm infants may benefit from this approach since large fluctuations in tissue oxygenation and perfusion increase the risk of mortality and morbidity.10
Recent studies investigating providing respiratory support in preterm infants with an intact umbilical cord deemed the procedure feasible.11–13 Although these studies monitored respiratory effort, HR and oxygen saturation (SpO2), these parameters were only measured after cord clamping. Moreover, the timing of cord clamping was always done at a fixed time point. We postulate that including the transitional status of the preterm infant as a key determinant for the timing of cord clamping may result in optimised cord clamping.
To enable PBCC in preterm infants, a new purpose-built resuscitation table (the Concord) was designed in a collaborative initiative of the Neonatal, Obstetric and Medical Engineering Departments of Leiden University Medical Centre (LUMC), according to the following criteria: (1) ability to provide full standard care for preterm stabilisation, (2) ability to monitor the infant, (3) prevention of kinking and stretching of the umbilical cord and (4) ability to proceed unhindered care for the mother. The aim of this study was to assess feasibility of the PBCC approach in preterm infants using this novel device.
We performed a prospective observational feasibility study at the LUMC from October 2016 to November 2017. The study was registered in the Netherlands National Trial Register (NTR6095), and Institutional Review Board approval (P16.146) was obtained prior to starting the study. An external safety board was not installed.
Infants were included if they were born between 26 and 35 weeks of gestational age (GA), and no complications other than preterm birth were expected. Informed consent was requested of all pregnant women who had been admitted to the obstetric department in our institution for imminent preterm birth. Inclusion started with preterm infants born between 30 weeks and 35 weeks of gestation. After including eight infants without any problems, we proceeded to include infants born between 26 weeks’ gestation and 35 weeks’ gestation. Initially, only infants born vaginally could be included, as precautions concerning sterility of the procedure in the operating room were still in progress. After 6 months of gaining experience and taking the appropriate precautions, infants born after caesarean section were included as well. Exclusion criteria were signs of placental abruption or placenta praevia, signs of severe fetal distress determined by the clinician and the necessity for an emergency caesarean section ordered to be executed within 15 min. In this feasibility study, the predefined goal was to include a total minimum of 16 infants, a minimum of 8 infants in both GA groups and a minimum of 5 infants born after caesarean section. All infants of parents who had consented to participate were allowed to be included even though the convenience sample was reached, which is why the final number exceeds our predefined samples.
A new custom-designed resuscitation table (the Concord, figure 1) was used to provide full standard of care for stabilisation of preterm infants at birth with an intact umbilical cord. The Concord is a mobile device, of which the platform can be adjusted to be positioned in very close proximity to the birth canal without stretching the umbilical cord. The platform contains a slit through which the umbilical cord can be placed. The table is provided with the same equipment for resuscitation as used in our standard resuscitation table, being a T-piece ventilator, radiant warmer, suctioning device, oxygen blender, heater and humidifier, and a heated resuscitation circuit. In addition, the Concord contains a respiratory function monitor (NewLifeBox Neo-RSD computer system; Advanced Life Diagnostics, Weener, Germany), depicting respiratory function, SpO2, HR and fraction of inspired oxygen (FiO2), as well as a video camera (Applied Biosignals, Weener, Germany). We obtained SpO2 and HR measurements using a pulse oximeter (Masimo Radical, Masimo Corporation, Irvine, California, USA). All resuscitation procedures were video recorded as is customary at our institution. The signals were digitised at 200 Hz using the NewLifeBox –R physiological recording system (Advanced Life Diagnostics) supported by Polybench physiological software (Applied Biosignals).
Prior to the start of the study all caregivers involved in labour care were trained in using the Concord, for which a standard operating procedure was written. All neonatal caregivers were trained and accredited for neonatal resuscitation. After each procedure details of birth and aspects of stabilisation were evaluated with the staff involved. Any safety issues or problems reported during the evaluation would be resolved before the next infant would be included.
The Concord was placed next to the woman’s bed before progression to the second stage of labour or next to the theatre table prior to the start of caesarean section. All equipment was checked and set up in advance. The standard resuscitation table and equipment were also prepared as fall back option. If the attending neonatologist or obstetrician considered that PBCC should not be performed or interrupted, the infant would be taken to the standard resuscitation table for (further) stabilisation.
The time of birth was defined as the moment when the infant was completely out of the birth canal. At this moment, the timer was started. To enable PBCC. the obstetrician held the infant after birth, and the woman was asked to lower her left leg. The platform of the Concord was then placed above the woman’s pelvis as close as possible to the birth canal to ensure that the umbilical cord was not stretched. The infant was placed on the platform and received stabilisation and heat loss prevention according to standard guidelines by the neonatal team, while the obstetrician assessed maternal condition and checked that the umbilical cord was not stretched nor kinked. After a vaginal delivery, parents were encouraged to touch and stimulate the infant during stabilisation. Infants were stabilised following the institutional newborn resuscitation guideline, starting with heat loss prevention, adequate positioning and tactile stimulation. Face mask ventilation breaths or sustained inflations were applied to inadequately breathing infants (initial FiO2: 21%–30%), followed by continuous positive airway pressure (CPAP) or a period of positive pressure ventilation (PPV) if necessary. Surfactant was not administered in the delivery room. As soon as the infant was considered stable, defined as the establishment of adequate breathing (average tidal volume ≥4 mL/kg) on CPAP, HR above 100 bpm and SpO2 above 25th percentile using FiO2 <0.4, the cord was clamped. Uterotonic drugs were administered immediately after cord clamping. Subsequently, the platform was pulled back and placed next to the bed of the woman, enabling physical contact with her infant. The infant was then transferred to the transport incubator and transported to the neonatal intensive care unit (NICU).
In case of twin birth, clamping of the first infant was performed according to PBCC or earlier if the second infant was about to be born. After clamping, the first infant was transferred to a standard resuscitation table, so the second infant could be stabilised using the Concord. Only the second infant was stabilised using the Concord when twins were born after caesarean section, due to logistical reasons.
The PBCC approach would be aborted immediately in case of: (1) the maternal condition requiring more work space for the obstetrical team, (2) full cardiac resuscitation of the infant or (3) excessive maternal blood loss as determined by the obstetrical team.
Outcome and safety parameters
The primary outcome was the ability to perform PBCC. Secondary outcomes were timing of cord clamping, HR and SpO2 in the first 10 min of life and important safety parameters: maternal blood loss, infant temperature at NICU admission and the incidence of polycythaemia or severe hyperbilirubinaemia requiring exchange transfusion. Maternal and infant baseline characteristics and short-term outcomes were collected.
Measurements of HR, SpO2 and FiO2 were obtained every 0.5 s and automatically averaged over a 2 s interval by pulse oximeter. Measurements were obtained until 10 min after delivery of the neonate. Data points were analysed if a good quality pulse oximeter signal was obtained without any alarm messages (low identification, low perfusion index and sensor off). HR and SpO2 measurements are displayed per minute, presented as median (IQR), starting from the first minute after birth. All measurements were averaged over a 6 s period around every whole minute. Measurements on HR and SpO2 are specifically reported for infants born <32 weeks’ GA, as these infants are most in need of resuscitation, and this GA group is most commonly used in literature.
HR measurements obtained around the time of cord clamping were reviewed (from 10 s before to 30 s after umbilical cord clamping). Additionally, we calculated the occurrence and proportion of time SpO2 measurements were below, within and above target ranges only for infants born <32 weeks’ GA, as defined by White et al. More specifically, at 3–4 min, 4–5 min and 5–10 min, the corresponding SpO2 target ranges were 70%–90%, 75%–90% and 80%–90%, respectively.7
Data were analysed using IBM SPSS Statistic V.24.0 software. Normally distributed data are presented as mean±SD, not-normally distributed data as median (IQR). Pearson correlation test was used to test associations between continuous variables. Friedmans analysis was performed to test for differences between measurements over a time period. Graphs were designed using GraphPad Prism 7.
A total of 118 parent couples were approached for study participation of which 82 couples consented. Thirty-seven infants were included (figure 2) with a mean GA of 30.9±2.4 weeks. Baseline characteristics are shown in table 1.
PBCC was successful in 33 infants (89.2%, table 2) but was not performed in four infants for three reasons: (1) in two births the neonatal team arrived too late in the labour room to use the Concord and perform PBCC; (2) in one birth the umbilical cord was too short and had to be clamped immediately; and (3) in one birth the attending obstetrician had clinical concerns to perform PBCC (figure 2). We observed a median (IQR) cord clamping time of 4:23 (3:00–5:11) min after birth (vaginal birth 4:20 (2:55–5:11) min and caesarean section 4:49 (3:56–5:37) min). Most infants (94.6%) received respiratory support for stabilisation. No infant needed intubation or surfactant during stabilisation. Further details on perinatal outcomes are presented in table 2. There was no significant correlation between the timing of cord clamping and GA (r=−0.161; p=0.37), maternal blood loss (r=−0.119; p=0.52) or infant admission temperature (r=-0,157; p=0.38).
Due to technical failure, measurements on HR, SpO2 and FiO2 were not stored in 7 out of 33 infants. Therefore, physiological parameters could be analysed in 26 infants. The first reliable measurements were obtained at a median (IQR) time of 81.3 (50.8–117.8) s after birth. Measurements are reported for infants born <32 weeks’ GA (measurements available for 20 out of 26 infants) and are listed for all infants in table 3.
Median (IQR) HR for infants born <32 weeks’ GA was 123 (89–148), 127 (96–137), 134 (112–148) and 144(128–155) at 1, 2, 3 and 5 min after birth (figure 3A, table 3). One infant experienced an HR <60 bpm at the third minute, which occurred simultaneously with mask reposition and was thought to be the effect of the nasal trigeminal reflex. Median (IQR) HR at time of cord clamping was 139 (127–151) bpm, and Friedmans analysis showed no significant changes in HR before, during or after cord clamping, χ2(2)=0.000, p=1.000 (figure 3B).
Measurements for median (IQR) SpO2 were 54% (46%–67%), 58% (47%–82%), 79% (57%–91%)%) and 92% (84%–97%)%) at 1, 2, 3 and 5 min (figure 3C, table 3), while median (IQR) FiO2 given was 0.30 (0.30–0.31), 0.30 (0.30–0.31), 0.31 (0.29–0.51) and 0.31 (0.25–0.97), respectively. At periods 3–4 min, 4–5 min and 5–10 min proportions of time spent below the SpO2 ‘target range’ for infants born <32 weeks’ GA were 25.3%, 15.9% and 2.3% with administered median (IQR) FiO2 of 0.32 (0.28–0.75), 0.31 (0.27–0.79) and 0.31 (0.26–0.48). Proportion of time spent above SpO2 value of 95% were 7.4%, 22.1% and 30.1%, respectively (table 4).
Short-term clinical outcomes are summarised in table 5. The majority of infants received phototherapy for hyperbilirubinaemia; none of the neonates needed exchange transfusion. Three infants (8.1%) met the criteria of polycythaemia.
This is the first clinical study performing PBCC in preterm infants, using the infant’s transitional status as the key determinant to direct the timing of cord clamping. Our results demonstrated that PBCC, when using the Concord, is feasible in a large majority of infants (89%). All failures occurred at the beginning of our project, suggesting a learning curve for involved caretakers in timely being prepared for the procedure and in adequately positioning the Concord. Feasibility was good when compared with recent studies (ranging from 59% to 100%) where respiratory support was started before cord clamping though using a different set-up.11–13 International guidelines recommend delaying cord clamping for at least 30–60 s in stable preterm infants and immediate cord clamping for compromised infants.14 All recent studies providing respiratory support prior to cord clamping used time based cord clamping at 60–120 s.11–13 Our PBCC approach led to considerably later cord clamping than reported in these studies using fixed time points, so infants potentially received a more complete placental transfusion. Yao et al demonstrated this would take at least 3 min.15 Moreover, the Concord made it possible for infants needing respiratory support to benefit from delaying cord clamping.
This is the first study to record physiological parameters HR and SpO2 continuously in the first minutes of life when performing PBCC in preterm infants. Previous studies have investigated the effects of ventilation prior to cord clamping,11–13 but HR and SpO2 were either not reported or were not recorded continuously, making it difficult to compare our findings with the literature. We observed that the HR of (very) preterm infants were higher and more stable than described so far for other methods of cord clamping.7 16 In addition, no bradycardia was observed after umbilical cord clamping. Recent studies have described that bradycardia often occurs in the first minutes after birth when immediate or DCC is performed.7 16 17 Indeed, White et al observed bradycardia in more than 50% of infants at 2 min after delivery, suggested by their median (IQR) HR of 88 (71–147) after immediate cord clamping. In our cohort, we rarely observed bradycardia (HR 123 (89–148)) and interestingly HR also remained stable around the moment of cord clamping.7 This suggests that cardiac output depended to a lesser extent on placental venous return, while pulmonary blood flow had successfully increased to compensate. It is striking that the HR was higher and the rate of increase was less steep in the first 2 min compared with the nomogram of preterm infants who needed no support and were <37 weeks of GA but received ICC.16 These observations correspond with the results of the experimental PBCC studies and imply that the preterm infants were haemodynamically stable, with a gradual transition and without adverse events.4 5
Median SpO2 of the infants in our study increased quickly in the first 4 min after birth to a stable level above 90%, while there was very little change in the median FiO2. Median SpO2 increased at a faster pace than previously described for preterm infants in whom ICC was performed. Duration of hypoxaemia was shorter at lower levels of oxygen supply within the first 10 min.7 8 18 Avoiding hypoxaemia, especially in the first minutes after birth, is pivotal since this is associated with lower HR and increased risk of intraventricular haemorrhage and death.19 In addition, the occurrence of hypoxaemia and bradycardia would often lead to more vigorous PPV, which can be associated with increased morbidity. Mian et al recently demonstrated that delivery of high tidal volume is associated with intraventricular haemorrhage.20 Although our infants spent a greater proportion of time above the SpO2 target ranges when compared with White et al,7 only a small proportion had SpO2 values >95% while lower levels of additional oxygen were given.
Clamping the umbilical cord without the occurrence of bradycardia and decreasing the duration of hypoxaemia is likely to be the effect of haemodynamic stability created by PBCC. Clamping the umbilical cord after lung aeration avoids decrease in left ventricular output, seen after immediate cord clamping, since the source of preload can easily switch from umbilical to pulmonary venous return.21 22 Furthermore, the increase in afterload seen after ICC, due to loss of the placenta’s low vascular resistance, is greatly mitigated during PBCC since the pulmonary circulation becomes an alternative route for systemic blood flow through the ductus arteriosus.23 24 This results in the preservation of stable cardiac output and therefore a stable supply of oxygenated blood, which explains the higher SpO2 values and absence of bradycardia after PBCC. Acknowledging that these observations are derived from experimental animal-based research, we think our results trigger further and ongoing collection of human data concerning primary physiological changes during newborn transition.25
No serious adverse events regarding the Concord or the PBCC procedure were reported during the study. Maternal blood loss and the incidence of polycythaemia were within normal ranges and were not associated with duration of cord clamping.26 27 All infants born before 32 weeks of gestation needed phototherapy, but no exchange transfusion was required. A potential draw back was the mean infant temperature at NICU admission of 36.0°C (range 34.5°C–37.6°C). Although comparable with temperatures reported in some previous DCC studies,28 29 heat loss prevention using the Concord was an important focus. During our project, extra effort was undertaken in additional education for involved caretakers to decrease the incidence of hypothermia, especially in infants delivered via caesarean section as they experienced more hypothermia. This was established by early activation of the radiant heater, thus adequately prewarming the platform and adjusting room temperature in the operating theatre. Furthermore, the incidence of NEC in our cohort was higher than expected, mainly due to monochorionic twins of 32 weeks’ GA both experiencing NEC stage 2a, recovering without surgery. Interestingly, two out of the three infants experiencing NEC did not receive PBCC and were clamped early.
Although the study was not powered for physiological measurements, and measurements were available in only 26 out of 37 infants, IQR for most measurements were small indicating little variability between individuals; therefore, one could conclude that this represents a good estimation of HR and SpO2 during PBCC. Although various studies have demonstrated electrocardiogram (ECG) to give a more rapid and accurate measure of HR,30 31 the use of pulse oximeter in our study was for pragmatic reasons. Whether it is better to use ECG (electrical activity) or pulse oximeter (pulse wave, indication that electrical activity is followed by a pulse wave arriving in the peripheral circulation), remains open for debate. Variation between measurements was largest in the first 3 min after birth, which is consistent with current reference ranges.16 32 This was a single-centre study, and a subsequent larger multicentre randomised controlled trial is planned. This will enable us to evaluate effectiveness of PBCC in other centres and compare data with a control group.
In summary, considering the success percentage in performing PBCC, a more stable HR and faster increase in oxygenation and the absence of reported serious adverse events, we consider the PBCC approach in preterm infants using the Concord feasible. This report is the first to describe real-time monitoring of HR and SpO2 during PBCC in very preterm infants, and measurements provide human data that seem to support most of the experimental findings. Importantly, HR remained stable around cord clamping, which makes it likely that PBCC may result in optimal timing of cord clamping and in optimal pulmonary and cardiovascular transition. Further physiological research and larger randomised clinical studies are required to establish effectiveness of PBCC in improving clinical benefits in this challenging group of infants.
EB and RK contributed equally.
Contributors EB, RK and ABtP wrote the ethics application, participated in the study design and coordination, trained the clinicians, collected and analysed the data and reviewed the literature. ASNV, FJCMK and SBH participated in the study design. EB, RK, ASNV, FJCMK, ABtP, SBH and PEV participated in design and development of the device. EB and RK wrote the first draft and submitted the article. All authors participated in reviewing the data and editing the manuscript. All authors have read and approved the final manuscript.
Funding ABtP is recipient of an NWO (Netherlands Organisation for Scientific Research) innovational research incentives scheme (VIDI 91716428). This project was sponsored by the Gisela Thier Fund (Leiden) and the Sophia Children’s Hospital Foundation (Rotterdam).
Competing interests None declared.
Patient consent Parental/guardian consent obtained.
Ethics approval Commissie Medische Ethiek van het Leids Universitair Medisch Centrum.
Provenance and peer review Not commissioned; externally peer reviewed.
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