Objective To investigate the variation in severe neonatal morbidity among very preterm (VPT) infants across European regions and whether morbidity rates are higher in regions with low compared with high mortality rates.
Design Area-based cohort study of all births before 32 weeks of gestational age.
Setting 16 regions in 11 European countries in 2011/2012.
Patients Survivors to discharge from neonatal care (n=6422).
Main outcome measures Severe neonatal morbidity was defined as intraventricular haemorrhage grades III and IV, cystic periventricular leukomalacia, surgical necrotizing enterocolitis and retinopathy of prematurity grades ≥3. A secondary outcome included severe bronchopulmonary dysplasia (BPD), data available in 14 regions. Common definitions for neonatal morbidities were established before data abstraction from medical records. Regional severe neonatal morbidity rates were correlated with regional in-hospital mortality rates for live births after adjustment on maternal and neonatal characteristics.
Results 10.6% of survivors had a severe neonatal morbidity without severe BPD (regional range 6.4%–23.5%) and 13.8% including severe BPD (regional range 10.0%–23.5%). Adjusted inhospital mortality was 13.7% (regional range 8.4%–18.8%). Differences between regions remained significant after consideration of maternal and neonatal characteristics (P<0.001) and severe neonatal morbidity rates were not correlated with mortality rates (P=0.50).
Conclusion Severe neonatal morbidity rates for VPT survivors varied widely across European regions and were independent of mortality rates.
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What is already known on this topic?
Mortality rates for very preterm infants differ across European regions.
Morbidity rates may vary, but cross-regional comparisons are difficult because of unstandardised definitions and patient populations.
Mortality rates could affect the prevalence of severe neonatal morbidity among survivors if selection due to mortality affects patient case mix.
What this study adds?
This study confirms wide variations in neonatal morbidity across European regions using common definitions and patient populations.
Adjusting for maternal and neonatal characteristics reduces the variation between regions, but does not explain it.
A trade-off between mortality and morbidity rates at the regional level is not observed.
Delivery before 32 weeks of gestation occurs in 1%–2% of births, contributing to significant mortality and morbidity.1 Advances in obstetric and neonatal care with more active management of these infants2 have increased survival in high-income countries.3–7 However, differences in mortality rates persist between and within countries,6 which may be partly explained by professional attitudes and decision-making regarding extremely preterm infants.6 Differences in maternal demographic and socioeconomic characteristics and the organisation and quality of obstetrical and neonatal care also contribute to mortality variations.8 9
Increased survival among very preterm infants may lead to higher risks of neonatal morbidity among survivors.10 11 Studies in the 1990s and 2000 provided conflicting reports; some found increasing neonatal morbidity as survival improved, while others reported no change.12 More recent studies show that improved survival did not result in increased rates of major neonatal morbidities, although rates of bronchopulmonary dysplasia (BPD) may increase as more extremely preterm infants survive.13–18
While repeated studies by the same research groups enable standardised methodologies and comparable results over time,13–15 comparing morbidity across settings is more challenging, as morbidity has been measured using multiple definitions and study populations. Consequently, in contrast to mortality, few comparable data exist concerning geographical variation in morbidity in very preterm populations.19 20 Quantifying trade-offs between mortality and morbidity is relevant in a cross-country context, as improved neonatal survival may result in higher rates of impairment. Even if morbidity rates do not increase, the number of surviving high-risk infants will be greater when mortality is lower. This information is important for planning for health, social and educational services.
Using a contemporary prospective, population-based, multiregional cohort of live births <32 weeks gestation, we aimed to describe the variation in severe neonatal morbidity in Europe using standardised definitions, and to assess the potential trade-offs between survival and subsequent morbidity.
The ‘Effective Perinatal Intensive Care In Europe’ (EPICE) cohort is a prospective study of all stillbirths and live births between 22+0 and 31+6 weeks gestation in 19 regions in 11 European countries that together have >850 000 births annually: Belgium (Flanders), Denmark (Eastern Region), Estonia (entire country), France (Burgundy, Ile-de-France and the Northern region), Germany (Hesse and Saarland), Italy (Emilia-Romagna, Lazio and Marche), the Netherlands (Central and Eastern region), Poland (Wielkopolska), Portugal (Lisbon and Northern region), Sweden (greater Stockholm area) and the UK (East Midlands, Northern, and Yorkshire and Humber regions).21 The regions were included because of their geographical and organisational diversity, their ability to implement the study protocol and sample size considerations. Data were collected on births over varying 12-month periods between April 2011 and September 2012, except in French regions (6-month study period).
Data were extracted from medical records in obstetric and neonatal units using standardised pretested forms. Gestational age (GA) was defined as the best obstetric assessment based on information on last menstrual period and antenatal ultrasounds, which are part of routine obstetric care in all regions. The completeness of inclusions was cross-checked against delivery unit registers or routine regional statistics. Infants were followed until discharge from hospital to home or into long-term care, or death.
Three of the regions, Saarland (Germany), Marche (Italy) and Burgundy (France), were excluded because they had fewer than 100 eligible infants and therefore the number of severe morbid events was low. These regions had mortality and morbidity rates similar to the cohort average. For the analysis of mortality, we included 7438 live births after exclusion of stillbirths (n=1554), terminations of pregnancy (n=787) and severe congenital anomalies (n=123). Infants with severe congenital anomalies were excluded because of differences in screening and termination policies across the regions22 and consequent effects on mortality and morbidity. For the analysis of morbidity, our primary study population was infants discharged home or into long-term institutional care (n=6422). We also computed morbidity rates among neonatal admissions and inhospital deaths to allow comparison with other studies and to assess selection into our sample.
Definition of outcomes
Severe neonatal morbidity was defined as a composite measure of cystic periventricular leukomalacia (cPVL), intraventricular haemorrhage (IVH grades III and IV), surgical necrotizing enterocolitis (NEC requiring surgical treatment or peritoneal drainage) or retinopathy of prematurity (ROP≥stage 3). Severe BPD was need for oxygen with the fraction of inspired oxygen (FiO2) >30% or need for respiratory support (mechanical ventilation and non-invasive ventilation) at 36 weeks of postmenstrual age among survivors to ≥36 weeks.23 As data on FiO2 were not collected in two regions in the UK, we chose not to include severe BPD in our primary composite, but analysed it separately. Inhospital mortality was death after live birth before discharge from neonatal care, including labour ward deaths.
Maternal, pregnancy and neonatal characteristics used to adjust for population differences are those that differ between regions, have an impact on mortality and morbidity and do not reflect obstetrical or neonatal care.9 These include maternal age; parity; multifetal pregnancy; pregnancy complications (pre-eclampsia, eclampsia/hemolysis elevated liver enzymes low platelet count), preterm premature rupture of membranes >12 hours; GA in completed weeks; small for gestational age (SGA) defined as a birth weight for GA≤10th percentile of intrauterine growth curves24; infant sex and presence of congenital anomalies. Congenital anomalies were those reportable to Eurocat,25 but not considered severe,9 as these were excluded. We also describe other perinatal care and health outcomes: delivery in a level III maternity unit using national definitions (yes/no), antenatal corticosteroids administration (any/no), mode of delivery (prelabour caesarean, intrapartum caesarean or vaginal delivery), 5 min Apgar score, initial respiratory support (none, nasal continuous positive airway pressure (nCPAP) or mechanical ventilation) and neonatal transport within 48 hours after birth.
We first described the prevalence of severe neonatal morbidity without and with severe BPD by region for surviving infants<32 weeks of GA and by GA subgroup (<28 weeks and 28–31 weeks of GA). Descriptive tables were also generated for neonatal admissions and inhospital deaths.
We then computed adjusted rates of mortality and severe neonatal morbidity (without and with severe BPD) taking into consideration maternal, pregnancy and infant characteristics. Adjusted rates were derived using logistic regressions following methods used to analyse mortality in the EPICE sample.9 For each region, the ratio of the observed to the expected number of events was calculated by using the estimated predicted probabilities and was then multiplied by the observed rate in the study population. We used the Wald test to assess the significance of region in unadjusted and adjusted models. The variation between regions was quantified through the SD of the mortality percentages for the regions, expressed as a coefficient of variation. To test associations between adjusted regional mortality and morbidity rates, we used Spearman rank correlations. We used a minimal adjustment model for each individual morbidity component, including only GA, because of the low number of cases.
Ethical approval and informed parental consent (active or passive, depending on each participating country’s national legislation) for data collection were obtained in each study region. In the French regions, EPICE was carried out as part of the EPIPAGE 2 study, and parents had to consent to all parts of data collection, leading to 6.4% of total births for which consent could not be obtained.
The mean GA was 28.4 weeks and mean birth weight was 1194 g (table 1); 31.5% were <10th percentile by intrauterine growth standards, while 31.2% were multiples; 86.3% received antenatal corticosteroids and 64.8% were born by caesarean section. Over one quarter of the sample had mothers 35 years of age or over and 56.2% had primparous mothers. The inhospital mortality rate was 13.6% for all infants<32 weeks, 33.5% for infants<28 weeks and 4.2% for infants 28 to 31 weeks.
Severe morbidity rates by GA groups
Overall, 10.6% of survivors had one or more severe neonatal morbidities without severe BPD and 13.8% had severe morbidity when severe BPD was included (table 2A). Severe NEC was the least prevalent of the morbidities (1.9%), followed by cPVL (3.2%), ROP≥stage3 (3.7%), IVH grades III and IV (3.9%) and severe BPD (5.5%). For morbidity without BPD, the regional range was from <8% in Ile-de-France, the Northern region in France and Sweden (greater Stockholm area), to 23.5% in Poland (Wielkopolska).
For infants born before 28 weeks of GA, severe neonatal morbidity rates without and with severe BPD were 26.8%, and 36.9%, respectively (table 2B). Severe BPD, ROP≥stage 3 and IVH grade III/IV were the most prevalent morbidities. Variation between regions was wide (16% to >40%) for the composite variables. For infants 28 to 31 weeks, morbidity rates were 5.3% without severe BPD and 7.0% with severe BPD (table 2C); IVH grades III and IV, cPVL and severe BPD were the most prevalent morbidities. Differences between regions were significant (P<0.001) across both subpopulations and composite indicators.
The overall morbidity rate was higher when calculated for neonatal admissions (online supplementary table S1). Among deaths, 28% had a diagnosis of at least one morbidity, principally IVH grade III/V (22.1%) and NEC (6.5%). Proportions were lower for other morbidities with later age at diagnosis (online supplementary table S2).
Supplementary file 1
Adjustment for maternal and neonatal characteristics
While several maternal and neonatal characteristics were associated with severe neonatal morbidity in univariable analyses, only GA, SGA, pre-eclampsia/eclampsia and congenital anomalies remained significant in multivariable analyses, as shown in table 3. This was the model used to compute the adjusted morbidity rates. Regional variation remained significant after adjustment (P<0.001). The coefficient of variation of regional rates decreased from 35.2% to 31.7% after adjustment (59.9% to 51.8% for infants 28–31 weeks and 28.5% to 27.4% for infants <28 weeks).
Association between regional rates of morbidity and mortality
Risk-adjusted regional mortality rates ranged from 8.4% to 18.8% (unadjusted 7.3% to 21.1%) for the overall sample, 19.4% to 45.4% (unadjusted 17.0% to 43.8%) for infants <28 weeks and 1.4% to 6.0% (unadjusted 1.5% to 7.5%) for those 28–31 weeks (online supplementary table S3). Figure 1 illustrates the absence of an association between in-hospital mortality and morbidity without BPD among survivors, as shown by the Spearman rho included with the figure. Spearman rank correlations for each individual severe morbidity did not reveal negative associations, but there was a positive association with severe IVH (table 4). Scatter plots for each morbidity are included in supplementary material (online supplementary figure S1–S3)
Our study reveals wide variability in severe neonatal morbidity rates for very preterm infants across European regions: from 6% to 24% for overall morbidity without severe BPD. We did not observe a trade-off between rates of mortality and severe neonatal morbidity among surviving infants. In fact, for individual morbidities, we observed higher rates of severe brain lesions in higher mortality regions. Adjustment for maternal and neonatal characteristics reduced the variation between regions, but regional differences remained significant.
Strengths and limitations
The strengths of our study are its population-based design and the use of a common and pretested protocol for medical record abstraction. The morbidities included in this study have well-established definitions. For BPD, we used the fraction of inspired oxygen at 36 weeks or respiratory support to define severe cases.23 Two regions did not collect information on FiO2 and thus we could not include severe BPD in our primary outcome variable. However, results associating morbidity with mortality were similar without and with severe BPD, although rates of severe neonatal morbidity increased from 10.6% to 13.8% with the latter. Our regional samples were not large enough to provide a high degree of precision for the prevalence of individual morbidities; variability due to random chance should be considered when comparing the results between regions. Finally, we did not have data on maternal socioeconomic status. However, while socioeconomic factors are major predictors of preterm birth risk and the health and development of very preterm children26 27 they have not been associated with inhospital mortality or morbidity.28 29
Comparison with other studies
Comparison of morbidity rates across studies is complicated because of the use of different definitions, GA cut-offs and population inclusion criteria. The US Neonatal Research Network centres reported rates of IVH grade III/IV of 15%, cPVL 4%, NEC (Bell stage 2–3) 10% and ROP, stage 3 or more, 12% among infants born at less than 28 weeks in 2008–2012 and surviving more than 12 hours after birth.13 A California population-based study of survivors born at 22–28 weeks using hospital data reported rates of 7.5% for IVH grade III/IV, 2.3% for PVL, 6.7 for NEC and 9.9% for ROP requiring surgery.30 Among survivors born at 28–31 weeks in the EPIPAGE 2 cohort in France in 2011,14 the rate of IVH grade III or more was 3.1%; cPVL 1.6%; ROP stage 3 or more 0.1%. Among survivors born at 28–31 weeks, severe BPD was 3.1% and NEC 3.2%. These results are within ranges in our study; some differences are due to use of neonatal intensive care unit admissions versus survivors, which leads to increased rates. Our results are also in line with recent findings from studies over time concluding that there is no obvious trade-off between mortality and severe morbidity rates among survivors.6 13
Differences in regional morbidity rates were attenuated, but persisted, after risk adjustment. Fewer maternal and neonatal characteristics were associated with morbidity compared with previous analyses of mortality.9 As risk factors differ for individual morbidities, adjustment for patient case mix may be more complex when morbidity is analysed as a composite. For instance, SGA is not a risk factor for IVH or PVL, but strongly affects BPD31; male sex is strongly related to IVH, but may be less important for other morbidities.32
In previous studies, regional differences have been attributed to variations in the use of interventions, such as tocolytics, administration of antenatal corticosteroids, delivery in tertiary centres and respiratory care.4 In this cohort, admission hypothermia varied widely between different regions and was associated with an almost twofold risk of neonatal mortality.33 However, there was no association with morbidity.33 Other evidence-based interventions in this cohort also showed a larger impact on mortality than morbidity.21 At the lowest GAs (22–24 weeks), initiation of active care may also differ between regions and influence the risks of mortality and neonatal morbidity.34 35
Implications for practice and policy
The variation in severe neonatal morbidity rates across regions suggests that reductions are possible with current medical knowledge. Very preterm birth leads to high costs for families, health services and societies due to life-long neurodevelopmental and other impairments.36 It is also associated with worse long-term health-related quality of life.37 Given the strong association between severe neonatal morbidity and adverse longer-term outcomes38 effective preventive actions represent a cost-effective use of finite healthcare resources. This focus is particularly important as the active management of extremely preterm infants becomes more widespread.35 Furthermore, we show the importance of having comparable international indicators of severe neonatal morbidity for surveillance, benchmarking and the large-scale research studies needed to improve our understanding of the determinants of severe neonatal morbidity.
AKEB and JZ contributed equally.
Contributors AKEB and JZ had access to the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: AKEB, JZ, AP, RFM, AvH, HV, LK, BNM, AF, JM, MC, SP, PVR, HB, ESD; acquisition, analysis or interpretation of data: AKEB, JZ, AP, RFM, AvH, HV, BM, AF, JM, MC, SP, PVR, HB, ESD and all authors in the Epice Research Group; drafting of the manuscript: AKEB, JZ, AP, RFM, AvH, HV, BM, AF, JM, MC, SP, PVR, HB, ESD; critical revision of the manuscript for important intellectual content and approval of final version of the manuscript: All authors (including investigators listed in Epice Research Group); statistical analysis: AKEB, JZ, AP. Final approval: all authors.
Funding The research leading to these results received funding from the European Union’s Seventh Framework Programme (FP7/2007-2013) under grant agreement n°259882. Additional funding is acknowledge from the following regions: France (French Institute of Public Health Research/Institute of Public Health and its partners the French Health Ministry, the National Institute of Health and Medical Research, the National Institute of Cancer, and the National Solidarity Fund for Autonomy; grant ANR-11-EQPX-0038 from the National Research Agency through the French Equipex Program of Investments in the Future; and the Prem Up Foundation); Poland (2012- 2015 allocation of funds for international projects from the Polish Ministry of Science and Higher Education); Sweden (Stockholm County Council (ALF-project and Clinical Research Appointment) and by the Department of Neonatal Medicine, Karolinska University Hospital), UK (funding for The Neonatal Survey from Neonatal Networks for East Midlands and Yorkshire and Humber regions).
Competing interests None declared.
Ethics approval French Advisory Committee on Use of Health Data in Medical Research and the French National Commission for Data Protection and Liberties. Also, ethics authorisation was obtained for each study in the participating regions.
Provenance and peer review Not commissioned; externally peer reviewed.
Data sharing statement Access to data in the EPICE cohort for researchers who are not members of the consortium is not currently possible, but EPICE is part of a H2020 project (RECAP, https://recap-preterm.eu/) to develop a Platform for data sharing. Please contact the corresponding author for more information.
Collaborators BELGIUM: Flanders (E Martens, G Martens, P Van Reempts); DENMARK: Eastern Region (K Boerch, A Hasselager, L Huusom, O Pryds, T Weber); ESTONIA (L Toome, H Varendi); FRANCE: Burgundy, Ile-de France and Northern Region (PY Ancel, B Blondel, A Burguet, PH Jarreau, P Truffert); GERMANY: Hesse (RF Maier, B Misselwitz, S Schmidt), Saarland (L Gortner); ITALY: Emilia Romagna (D Baronciani, G Gargano), Lazio (R Agostino, D DiLallo, F Franco), Marche (V Carnielli), M Cuttini; ; NETHERLANDS: Eastern & Central (C Koopman-Esseboom, A Van Heijst, J Nijman); POLAND: Wielkopolska (J Gadzinowski, J Mazela); PORTUGAL: Lisbon and Tagus Valley (LM Graça, MC Machado), Northern region (Carina Rodrigues, T Rodrigues), H Barros; SWEDEN: Stockholm (AK Bonamy, M Norman, E Wilson); UK: East Midlands and Yorkshire and Humber (E Boyle, ES Draper, BN Manktelow), Northern Region (AC Fenton, DWA Milligan); INSERM, Paris (J Zeitlin, M Bonet, A Piedvache).
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