Cloning and sequencing of the 5' region of the human glucose-6-phosphatase gene: transcriptional regulation by cAMP, insulin and glucocorticoids in H4IIE hepatoma cells

D Schmoll; B B Allan; A Burchell

doi:10.1016/0014-5793(96)00224-4

Cloning and sequencing of the 5' region of the human glucose-6-phosphatase gene: transcriptional regulation by cAMP, insulin and glucocorticoids in H4IIE hepatoma cells

FEBS Lett. 1996 Mar 25;383(1-2):63-6. doi: 10.1016/0014-5793(96)00224-4.

Authors

D Schmoll¹, B B Allan, A Burchell

Affiliation

¹ Department of Obstetrics and Gynaecology, Ninewells Hospital and Medical School, University of Dundee, UK.

PMID: 8612793
DOI: 10.1016/0014-5793(96)00224-4

Abstract

We have cloned and sequenced the first 1.2 kb of the 5' region of the human glucose-6-phosphatase gene. Transfection of H4IIE hepatoma cells with the 1.2 kb fragment fused to a luciferase reporter gene demonstrated both basal and hormone responsive luciferase activity. Dexamethasone increased and insulin decreased luciferase activity. Insulin and dibutyryl cyclic AMP both significantly decreased activity in the presence of dexamethasone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Bucladesine / pharmacology*
Cloning, Molecular
Dexamethasone / pharmacology*
Gene Expression Regulation, Enzymologic / drug effects*
Genes, Reporter
Glucose-6-Phosphatase / genetics*
Humans
Insulin / pharmacology*
Liver Neoplasms, Experimental
Molecular Sequence Data
Promoter Regions, Genetic / genetics
Rats
Transcription, Genetic
Transfection / genetics
Tumor Cells, Cultured

Substances

Insulin
Bucladesine
Dexamethasone
Glucose-6-Phosphatase

Associated data

GENBANK/X96937