Abstract
After treatment with the culture supernatant of Bacteroides fragilis or with an endo-beta-galactosidase from Escherichia freundii, the human red blood cells are Tk-activated, i.e. agglutinable by BS II lectin and their blood group I and i activities are reduced. These results suggest that B. fragilis produces an endo-beta-galactosidase activity and that the main antigenic determinant of Tk polyagglutinable red cells is a terminal N-acetyl-glucosamine residue.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Agglutination / drug effects*
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Bacteroides fragilis / enzymology
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Bacteroides fragilis / immunology
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Erythrocytes / drug effects
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Erythrocytes / immunology
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Erythrocytes / metabolism
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Escherichia / enzymology
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Galactosidases / pharmacology*
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Glycoside Hydrolases*
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beta-Galactosidase / metabolism
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beta-Galactosidase / pharmacology*
Substances
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Galactosidases
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Glycoside Hydrolases
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keratan-sulfate endo-1,4-beta-galactosidase
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beta-Galactosidase