Abstract
Polyclonal anti-idiotypic antibodies were raised in mice against anti-Leu3a, a mouse monoclonal anti-human T4 (CD4) antibody that blocks the in-vitro binding of human immunodeficiency virus (HIV) to the CD4 molecule. The anti-idiotypes recognized anti-Leu3a but not OKT4, an anti-human T4 antibody that does not inhibit HIV binding to CD4. The anti-idiotypes specifically reacted with the HIV envelope glycoprotein in solid-phase immunoassays. More importantly, the anti-idiotypes neutralised three distinct isolates of HIV-1 and one isolate of HIV-2 in a syncytial inhibition assay. These results have implications for a potential AIDS vaccine of anti-CD4 preparations to induce an anti-idiotypic response with the capacity to bind HIV at its receptor site.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acquired Immunodeficiency Syndrome / prevention & control*
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Animals
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Antibodies, Monoclonal / analysis
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Antibodies, Monoclonal / immunology*
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Antigens, Differentiation, T-Lymphocyte / immunology
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Antigens, Viral / immunology
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Epitopes / analysis
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Epitopes / immunology*
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HIV / immunology*
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HIV Antigens
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Immunoglobulin Idiotypes / immunology
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Mice
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Mice, Inbred BALB C
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Neutralization Tests
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Radioimmunoassay
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Recombinant Proteins / immunology
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Rosette Formation
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Viral Envelope Proteins / immunology
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Viral Vaccines / immunology*
Substances
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Antibodies, Monoclonal
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Antigens, Differentiation, T-Lymphocyte
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Antigens, Viral
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Epitopes
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HIV Antigens
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Immunoglobulin Idiotypes
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Recombinant Proteins
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Viral Envelope Proteins
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Viral Vaccines