Elsevier

Genomics

Volume 13, Issue 3, July 1992, Pages 622-629
Genomics

Isolation and chromosomal assignment of 100 highly informative human simple sequence repeat polymorphisms

https://doi.org/10.1016/0888-7543(92)90133-DGet rights and content

Abstract

One hundred highly informative simple sequence repeat (SSR) polymorphisms have been isolated and mapped to specific human chromosomes by somatic cell hybrid analysis. These markers include 97 (CA)n, 2 (AGAT)n, and a single (AACT)n repeat. All the SSRs have heterozygosities > 0.50 and can be amplified using identical PCR conditions. At least one SSR was detected on every chromosome, except for chromosomes 22 and Y. The frequency of (CA)n repeats on each chromosome was proportional to the relative chromosomal length, except for chromosome 15, on which a substantial excess of markers was identified.

Reference (28)

  • WilkieP.J. et al.

    Linkage map of human chromosome 9 microsatellite polymorphisms

    Genomics

    (1992)
  • BotsteinD. et al.

    Construction of a genetic linkage map in man using restriction length polymorphisms

    Am. J. Hum. Genet.

    (1980)
  • ChurchG.M. et al.

    Genomic sequencing

  • ChurchG.M. et al.

    Multiplex DNA sequencing

    Science

    (1988)
  • Cited by (0)

    1

    Present address: Collaborative Research, Inc., 2 Oak Park, Bedford, MA 01730.

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