Regulation of diacylglycerol kinase in rat brain membranes by docosahexaenoic acid

doi:10.1016/0304-3940(94)90961-X

Neuroscience Letters

Volume 179, Issues 1–2, 26 September 1994, Pages 171-174

https://doi.org/10.1016/0304-3940(94)90961-X Get rights and content

Abstract

The effect of docosahexaenoic acid (DHA) on the diacylglycerol kinase (DG kinase) activity in rat brain membranes was investigated. DHA at 500 μM concentration, stimulated the enzyme activity by about 2 fold. This effect was concentration-and time-dependent and was observed after very short periods of incubation (one min). DHA stimulation of DG kinase was observed only with rat brain membranes, and not with rat brain cytosol or rat liver membranes. Treating the rat brain membranes with phospholipase A₂ which released free fatty acids including DHA, significantly stimulated the DG kinase activity. It is concluded that DHA through its stimulatory effect on DG kinase may regulate the signalling events in growth-related situations in the brain such as synaptogenesis.

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Regulation of diacylglycerol kinase in rat brain membranes by docosahexaenoic acid

Abstract

Brain Res.

Int. J. Biochem.

Prog. Lipid Res.

Biochim. Biophys. Acta

Concerted CMP-dependent [3H]inositol labelling of phosphoinositides and agonist activation of phospholipase C in rat brain cortical membranes

J. Neurochem.

Lipid composition of synaptic plasma membrane isolated from rat brain by zonal centrifugation

Biochemistry

Chemistry and metabolism of lipids in the vertebrate retina

Prog. Lipid Res.

The role of phosphoprotein B-50 in phosphoinositide metabolism in brain synaptic plasma membranes

Concerted CMP-dependent [³H]inositol labelling of phosphoinositides and agonist activation of phospholipase C in rat brain cortical membranes