A sensitive method for the determination of plasma catecholamines using liquid chromatography with electrochemical detection☆
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2018, Cell MetabolismCitation Excerpt :This means that when methods are changed/altered, adjustments are made to allow reliable comparisons between the same variables obtained at different time points. Plasma catecholamines were determined by the accredited laboratory using liquid chromatography followed by electrochemical detection (Hallman et al., 1978). For adrenaline, the detection limit was 0.1 nmol/l (Wahrenberg et al., 1989) and the coefficient of variation was 8% (Arner et al., 1981).
Development of a novel flexible polymer-based biosensor platform for the thermal detection of noradrenaline in aqueous solutions
2017, Chemical Engineering JournalCitation Excerpt :Due to its relevance as a biomarker, various analytical techniques have been employed to determine noradrenaline concentrations in biological samples. The most common methods include chromatographic [18,19] and electrochemical biosensor techniques [20–22]. Electrochemical methods are inexpensive and fast compared to chromatographic measurements, but applications are limited due to poor selectivity and interference of other metabolites present in biological samples.
Determination of catecholamines in plasma and urine
2013, Best Practice and Research: Clinical Endocrinology and MetabolismCitation Excerpt :Alpha-methyldopa and labetalol are classical sources of interferences observed when fluorescence detection is used [7,8]. In the early 80s, electrochemical detection (ECD) became popular since it allowed quantitating catecholamines in plasma from patients [9,10]. However, interference of labetalol causes spuriously raised E levels [11].
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2012, Metabolism: Clinical and ExperimentalCitation Excerpt :Plasma β-OH-butyrate was analyzed enzymatically by spectrophotometer [18]. Plasma adrenaline and noradrenaline were determined by high-performance liquid chromatography and electrochemical detection [19]. Serum lactate was measured fluorometrically [20].
Luminescent lanthanide sensors
2011, Advances in Inorganic ChemistryCitation Excerpt :For all these reasons, rapid detection of CAs in blood and urine can provide vital information that might aid in more efficient diagnosis as well as more effective treatment of various disease states. Current methods of detection for CAs in biological fluids (urine, plasma, and serum) involve chromatographic separation coupled to either electrochemical (119,120) or optical (121)techniques. However, most optical methods rely purely on the native fluorescence of CAs (λex ∼ 280 nm, λem ∼ 310 nm) (122), which have small Stokes shifts and suffer from signal losses due to reabsorption; others involve pre- or postcolumn derivatization with various fluorophores, such as naphthalene-2,3-dicarboxaldehyde (123), 1,2-diphenylethylenediamine (124,125), or fluorescamine (126).
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Supported by grants from the Swedish Medical Research Council (04X-2295, 17X-2330), Karolinska Instituet and T. och R. Söderbergs Stiftelse.