A sensitive, direct colorimetric assay of serum iron using the chromogen, nitro-PAPS
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2023, Free Radical Biology and MedicineApplication of HPLC to measure vanadium in environmental, biological and clinical matrices
2020, Arabian Journal of ChemistryCitation Excerpt :In aqueous solution the chelating reagent 2-(5-Nitro-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]-phenol abbreviated nitro-PAPS was used. This ligand forms very strong complexes with vanadium and particularly vanadium(V) which is the most likely form of vanadium in aqueous solutions at low concentration and near the neutral pH range (Makino et al., 1988). The separation of vanadium-nitro-PAPS was conducted using RP-HPLC in the ion pair mode (IP-RP-HPLC) which is a very common method for analysis of vanadium in water.
Status of malondialdehyde, catalase and superoxide dismutase levels/activities in schoolchildren with iron deficiency and iron-deficiency anemia of Kashere and its environs in Gombe State, Nigeria
2019, HeliyonCitation Excerpt :Human MDA ELISA kit (Catalogue No: EKHU-0372) was purchased from Melsin Medical Co., Limited, China. Iron in the serum was calorimetrically measured by the Nitro-PAPS method (Makino et al., 1988) – Monoreagent at 578 nm. Hemoglobin and hematocrit levels/counts in the blood were quantitatively analyzed using Sysmex hematology analyzer machine.
Age-relevant renal effects of cadmium exposure through consumption of home-harvested rice in female Japanese farmers
2013, Environment InternationalCitation Excerpt :We calculated estimated glomerular filtration rate (eGFR), as an indicator for renal glomerular function, using the equation for female Japanese: eGFR (mL/min/1.73 m2) = 194 × serum creatinine− 1.094 × age− 0.287 × 0.739 (Matsuo et al., 2009). For a subject with cadmium nephropathy, we additionally examined red blood cell count, hemoglobin, and hematocrit in peripheral blood collected with EDTA by the automated hematology analyzer SE-9000 (Sysmex, Hyogo, Japan), inorganic phosphorus, iron, ferritin, and erythropoietin in serum by the enzymatic method (Berti et al., 1988), colorimetric method (Makino et al., 1988), chemiluminescent immunometric assay (Dupuy et al., 2009), and radioimmunoassay (Sherwood and Goldwasser, 1979), respectively, inorganic phosphorus in urine by the enzymatic method, and bone mineral density in the non-dominant forearm using dual energy X-ray absorption (DTX-200, Osteometer MediTech, Inc., Hawthorne, CA, USA). Tubular reabsorption of phosphorus (TRP) was calculated using the following formula: (1 − urinary phosphorus × serum creatinine/urinary creatinine × serum phosphorus) × 100 (%).
Distribution of trace elements like calcium, copper, iron and zinc in serum samples of colon cancer - A case control study
2011, Journal of King Saud University - ScienceMethods for Studying Iron Metabolism in Yeast Mitochondria
2007, Methods in Cell BiologyCitation Excerpt :Determine the absorbance at 585 nm against the blank sample in quartz cuvettes. The absorption coefficient ɛ585 nm is ∼94,000 M−1 cm−1 (Makino et al., 1988). The same protocol may also be used for the iron chelator Ferene (3‐(2‐pyridyl)‐5,6‐bis(2‐[5‐furylsulfonic acid])‐1,2,4‐triazine).