Elsevier

Clinica Chimica Acta

Volume 173, Issue 2, 15 April 1988, Pages 183-191
Clinica Chimica Acta

A new microtechnique for the analysis of the human hepatic microsomal glucose-6-phosphatase system

https://doi.org/10.1016/0009-8981(88)90256-2Get rights and content

Abstract

A microtechnique has been developed which enables a complete kinetic analysis of the human hepatic microsomal glucose-6-phosphatase system to be carried out on microsomes isolated from very small liver samples. Complete or partial deficiencies of any of the proteins of the glucose-6-phosphatase system resulting in Type 1a, 1b, 1c or 1d glycogen storage disease can therefore be diagnosed using hepatic needle biopsy samples, whereas previous methods of diagnosis needed large wedge biopsy samples requiring laparotomy.

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    In isolated hepatocytes, this Ca2+-ATPase—dependent on magnesium, calcium, and calmodulin [15]—is also indirectly stimulated by glucose-6-phosphate, which provides intravesicular phosphate anions as a result of the enzymatic hydrolysis by glucose-6-phosphatase [43]. In fact, it is believed that the location of glucose-6-phosphatase in the luminal surface of the ER represents a physiological adaptation, by which increasing of glucose-6-phosphate levels—as a product of glycogenolysis stimulated by Ca2+-mobilizing hormones—ameliorates the calcium reuptake by the ER [40,43]. Another factor contributing to the lower liver microsomal Ca2+-ATPase activity of obese rats should be alterations in the fatty acid composition of membrane [50].

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