Elsevier

Clinica Chimica Acta

Volume 151, Issue 3, 15 October 1985, Pages 293-300
Clinica Chimica Acta

Benzoylalanine: detection and identification of an alanine conjugate with benzole acid in hyperammonemic patients treated with sodium benzoate

https://doi.org/10.1016/0009-8981(85)90092-0Get rights and content

Abstract

Urine of four hyperammonemic patients who were treated with large amounts of sodium benzoate were analyzed. Gas chromatographic and gas chromatograph-mass spectrometric analysis showed the presence of large amounts of benzoylalanine in addition to much hippuric acid. Benzoylalanine was identified by comparison with an authentic standard by means of its mass spectrum and the gas chromatographic retention time of its trimethylsilyl derivative. This abnormal metabolite is thought to be derived from the conjugation of benzoyl CoA with alanine. It is suggested that the excretion of benzoylalanine results from a reduction in the liver free glycine level caused by hippuric acid synthesis.

Cited by (13)

  • Metabolic profiling with gas chromatography-mass spectrometry and its application to clinical medicine

    1986, Journal of Chromatography B: Biomedical Sciences and Applications
View all citing articles on Scopus
View full text