A rapid and simple method for the measurement of plasma 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 using Sep-Pak C18 cartridges and a single high-performance liquid Chromatographie step
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LC-MS/MS analysis of vitamin D<inf>3</inf> metabolites in human serum using a salting-out based liquid-liquid extraction and DAPTAD derivatization
2021, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesCitation Excerpt :A range of techniques have been reported for the quantification of 25(OH)D3 in plasma or serum. One of the earliest techniques was competitive binding assay utilizing vitamin D binding protein [8] followed by high performance liquid chromatography (HPLC) [8–12] then radioimmunoassay (RIA) [8]. Those techniques were not devoid of shortcomings, for instance, competitive binding assay and RIA potentially suffered from overestimation due to cross reactivity with other vitamin D metabolites [6,8] while the more selective HPLC had lower sensitivity, low throughput, was costly and was technically demanding [8].
Measurement of circulating 25-hydroxyvitamin D: A historical review
2015, Practical Laboratory MedicineCitation Excerpt :Eisman et al. [60] published the 1st HPLC method for the measurement of 25OHD in 1978, followed within a year by Gilbertson et al. [61] and Jones [62]. Variants of these initial methods have been published until very recently [63–76]. As can be appreciated, although the HPLC-based methods were able to separate 25OHD2 from 25OHD3, the authors used either a single in-house or commercially available labeled 25OHD3 as internal standard or even surrogate molecules (retinyl acetate, docecanophenone, derivatised 25-hydroxydehydrocholesterol, 1α-OHD) to monitor the recovery of 25OHD, although reporting in most case concentrations for both isomers.
Determination of vitamin D<inf>3</inf> metabolites: State-of-the-art and trends
1999, Journal of Pharmaceutical and Biomedical AnalysisVitamin D treatment and bone mineral density in the aged
1996, MaturitasDetermination of 25-hydroxyvitamin D<inf>3</inf> in human plasma using a non-radioactive tetranorvitamin D analogue as an internal standard
1995, Journal of Chromatography B: Biomedical Sciences and ApplicationsAutomation of liquid chromatographic techniques for biomedical analysis
1989, Journal of Chromatography B: Biomedical Sciences and Applications