An improved method for routine determination of vitamin D and its hydroxylated metabolites in serum from children and adults
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Cited by (45)
Measurement of circulating 25-hydroxyvitamin D: A historical review
2015, Practical Laboratory MedicineCitation Excerpt :It measured 25OHD3 and 25OHD2 equally and exhibited a 100% cross-reactivity for 24,25(OH)2D. Parviainen et al. [46] published in 1981, a method based on HPLC separation of vitamin D metabolites and their subsequent measurement with a CBPA for 25OHD and 24,25(OH)2D, and a vitamin D-receptor assay for 1α,25(OH)2D. Although the recovery of the labeled metabolites was relatively low, the coefficient of variation (CV) was <10% for 25OHD.
Effect of unkilned and large amounts of oats on nutritional state of celiac patients in remission
2009, e-SPENCitation Excerpt :The following analyses were made using routine clinical laboratory methods: blood hemoglobin, serum iron, serum ferritin (immunoluminometric assay), vitamin B12 (radioisotope dilution), calcium, magnesium, cholesterol, HDL- cholesterol, triglycerides and folic acid in erythrocytes (E-folate) (saturation analysis). Serum vitamin D metabolite was assayed as described by Parviainen et al.19 Vitamins A and E were determined by using HPLC with ultraviolet detection by the method of De Leenheer (1979) as modified by Parviainen and Koskinen.20 Statistical analyses were performed using the SPSS statistical program (SPSS Inc., Chicago, IL).
Scintillation proximity assay for calcitriol in serum without high pressure liquid chromatography
1993, Clinica Chimica Acta