Elsevier

Brain Research

Volume 92, Issue 1, 4 July 1975, Pages 73-87
Brain Research

DNA, ganglioside and sulfatide in brains of rats given corticosterone in infancy, with an estimate of cell loss during development

https://doi.org/10.1016/0006-8993(75)90528-4Get rights and content

Summary

Evidence of a postnatal loss of cerebral cells has been presented, based on counts of the activity of cerebral DNA following labeling with [3H]thymidine on embryonic day 14. In otherwise untreated rats, the loss was about 15% of the labeled cells between postnatal days 3 and 15. There was no further loss of activity thereafter. Littermates given corticosterone at day 3 showed little additional loss of labeled cells. Ganglioside NANA (N-acetylneuraminic acid) was reduced in proportion to the reduction in cerebral weight in the corticosterone treated rats. Sulfatide was reduced more, so that the concentration was 11% below that of the controls. It is concluded that the deficit in DNA after postnatal corticosterone treatment must be due primarily to an irreversible suppression of DNA synthesis, involving mainly glia. The reduction in gangliosides may represent a deficit in the growth of neuronal processes, leading to a reduction in the amount of neuropil, and perhaps contributing to a decrease in the number or size of myelinated axons.

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    Dr. Evelyn Howard died after this research was completed. Her research is currently being carried on by Dr. David S. Olton. All questions that would normally be addressed to Dr. Howard should be referred to: Dr. David S. Olton, Department of Psychiatry and Behavioral Sciences, The Johns Hopkins School of Medicine, Baltimore, Md. 21205, U.S.A.

    *

    Present address: Biological Sciences Research Center and Department of Biochemistry, University of North Carolina, Chapel Hill, N. C. 27514, U.S.A.; please send reprint requests to this address.

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