A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid)] has been synthesized and shown to be useful for determination of sulfhydryl groups.
Several applications have been made to show its usefulness for biological materials.
A study of the reaction of this disulfide with blood has produced some evidence for the splitting of disulfide bonds by reduced heme.
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