Abstract
Early detection of delayed cerebral energy failure may be important in the prevention of reperfusion injury of the brain after severe perinatal hypoxia-ischaemia (HI). This study investigated whether monitoring of the redox state of cytochrome aa3 (Cytaa3) with near infrared spectroscopy (NIRS) after severe perinatal asphyxia may allow us to detect early a compromised energy metabolism of the developing brain. We therefore correlated serial Cytaa3 measurements (to estimate mitochondrial oxygenation) simultaneously with the 31phosphorous-magnetic resonance spectroscopy (31P-MRS)-measured phosphocreatin/inorganic phosphate (PCr/Pi) ratio (to estimate cerebral energy reserve) in newborn piglets before and after severe hypoxia-ischaemia. The animals were treated upon reperfusion with either allopurinol, deferoxamine, or 2-iminobiotin or with a vehicle to reduce post-HI reperfusion injury of the brain. Four sham-operated piglets served as controls. Before HI, the individual Cytaa3 values ranged between −0.02 and 0.71 μmol/L (mean value: −0.07) relative to baseline. The pattern over post-HI time of the vehicle-treated animals was remarkably different from the other groups in as far Cytaa3 became more oxidised from 3 h after start of HI onwards (increase of Cytaa3 as compared with baseline), whereas the other groups showed a significant reduction over time (decrease of Cytaa3 as compared with baseline: allopurinol and deferoxamine) or hardly any change (2-iminobiotin and sham-operated piglets). Vehicle-treated piglets showed a significant reduction in PCr/Pi at 24 h after start of HI, but the cerebral energy state was preserved in 2-iminobiotin-, allopurinol- and deferoxamine-treated piglets. With severe reduction in PCr/Pi-ratio, major changes in the redox-state of Cytaa3 also occurred: Cytaa3 was mostly either in a reduced state (down to −6.45 μmol/L) or in an oxidised state (up to 6.84 μmol/L) at these low PCr/Pi ratios. The positive predictive value (PPV) of Cytaa3 to predict severe reduction of the PCr/Pi ratio was 42%; the negative PPV was 87%. A similar relation was found for Cytaa3 with histologically determined loss of neurons.
Similar content being viewed by others
Abbreviations
- Cytaa 3 :
-
Cytochrome aa3
- HI :
-
Hypoxia-ischaemia
- NIRS :
-
Near infrared spectroscopy
- 31 P-MRS :
-
31Phosphorous magnetic resonance spectroscopy
- PCr/Pi :
-
Phosphocreatin/inorganic phosphate
References
Astrup J (1982) Energy-requiring cell functions in the ischemic brain. J Neurosurg 56:482–489
Blumberg RA, Cady EB, Wigglesworth JS, McKenzie JE, Edwards AD (1997) Relation between delayed impairment of cerebral energy metabolism and infarction following transient focal hypoxia-ischaemia in the developing brain. Exp Brain Res 113:130–137
Brazy JE, Lewis DV (1986) Changes in cerebral blood volume and cytochrome aa3 during hypertensive peaks in preterm infants. J Pediatr 108:983–987
Brunori M, Antonini E, Wilson MT (1981) Cytochrome C oxidase: an overview of recent work. In: Sigel H (ed) Metal ions in biological systems. Marcel Dekker, New York, pp187–228
Cooper CE, Delpy DT, Nemoto EM (1998) The relation of oxygen delivery to absolute haemoglobine oxygenation and mitochondrial cytochrome oxidase redox state in the adult brain: a near-infrared spectroscopy study. Biochem J 332:627–632
Dimlich R, Showers MJ, Shipley MT (1990) Densitometric analysis of cytochrome oxidase in ischemic rat brain. Brain Res 516:181–191
Jobsis FF (1977) Noninvasive infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters. Science 198:1264–1267
Jobsis FF, Keizer JH, La Manna JC, Rosenthal M (1977) Reflectance spectrophotometry of cytochrome aa3 in vivo. J Appl Physiol 43:858–872
Lorek A, Takei Y, Cady EB, Wyatt JS, Penrice J, Edwards AD, Peebles D, Wylezinska M, Owen-Reece H, Kirkbride V, Cooper C, Aldridge RF, Roth SC, Brown G, Delpy DT, Reynolds EOR (1994) Delayed (“secondary”) cerebral energy failure after acute hypoxia-ischaemia in the newborn piglet: continuous 48-hour studies by phosphorous magnetic resonance spectroscopy. Pediatr Res 36:699–706
Matsumoto H, Oda T, Hossain MA, Yoshimura N (1996) Does the redox state of cytochrome aa3 reflect brain energy level during hypoxia? Simultaneous measurements by near infrared spectroscopy and 31P nuclear magnetic resonance spectroscopy. Anesth Analg 83:513–5189
Nelson C, Silverstein FS (1994) Acute disruption of cytochrome oxidase activity in brain in a perinatal rat stroke model. Pediatr Res 36:12–19
Peeters-Scholte C, Koster J, Veldhuis W, van Den Tweel E, Zhu C, Blomgren K, Bar D, Van Buul-Offers S, Hagberg H, Nicolay K, van Bel F, Groenendaal F (2002) Neuroprotection by selective nitric oxide synthase inhibition at 24 hours after perinatal hypoxia-ischaemia. Stroke 33:2304–2310
Pryds O, Greisen G, Skov LL, Friiss-Hansen B (1990) Carbon dioxide-related changes in cerebral blood volume and cerebral blood flow in mechanically ventilated preterm neonates: comparison of near infrared spectrophotometry and 133Xenon clearance. Pediatr Res 27:445–449
Reynolds EOR, Wyatt JS, Azzopardi D, et al (1988) New non-invasive methods for assessing brain oxygenation and haemodynamics. Brit Med Bull 44:1052–1075
Thomiley MS, Wickramasinghe YABD, Rolfe P (1988) Near infrared spectroscopy: a new technique for the non-invasive monitoring of tissue and blood oxygenation in vivo. Biochem Soc Trans 16:978–790
Thomiley MS, Wickramasinghe YABD, Rolfe P (1993) Near infrared spectroscopy: a new technique for the non-invasive monitoring of tissue and blood oxygenation in vivo. Biochem Soc Trans 16:978–789
Tsuji M, Naruse H, Volpe J, Holtzman D (1995) Reduction of cytochrome aa3 measured by near-infrared spectroscopy predicts cerebral energy loss in hypoxic piglets. Pediatr Res 37:253–259
Wagner KR, Kleinholz M, Myers RE (1990) Delayed onset of neurologic deterioration following anoxia/ischaemia coincides with appearance of impaired brain mitochondrial respiration and decreased cytochrome oxidase activity. J Cereb Blood Flow Metab 10:417–423
Wickramasinghe YABD, Rolfe P, Palmer K, Spencer SA (1995) Investigation of neonatal brain cytochrome redox by near infrared spectroscopy. Dev Brain Res 89:307–308
Wolf M, Evans P, Bucher HU, Dietz V, Keel M, Strebel R, von Siebenthal K (1997) Measurement of absolute cerebral haemoglobin concentration in adults and neonates. Adv Exp Med Biol 428:219–227
Wolf M, Keel M, Dietz V, von Siebenthal K, Bucher HU, Baenziger O (1999) The influence of a clear layer on near-infrared spectrophotometry measurements using a liquid neonatal head phantom. Phys Med Biol 44:1743–1753
Wyatt JS, Edwards AD, Azzopardi D, Reynolds EOR (1989) Magnetic resonance and near infrared spectroscopy for investigation of perinatal hypoxic-ischaemic brain injury. Arch Dis Child 64:953–996
Van Der Zee P, Cope M, Arridge SR, Delpy DT, Edwards AD, Reynolds EOR (1992) Experimentally measured optical pathlengths for the adult head, calf, and forearm and the head of the newborn infants as a function of inter optode spacing. Adv Exp Med Biol 316:143–155
Acknowledgements
We thank Tyco Healthcare, Pleasanton, CA, USA, for putting a Nellcor NPB 290 oxymeter at our disposal. We also thank the biotechnicians of the Central Laboratory Animal Institute of the Utrecht University for their enthusiastic help during the experiments. Finally, we thank Kees Braun, Wouter Veldhuis and Prof. K. Nicolay for their help during the MRI/MRS experiments and Prof. P.R. Bär for critically reviewing the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Peeters-Scholte, C., van den Tweel, E., Groenendaal, F. et al. Redox state of near infrared spectroscopy-measured cytochrome aa3 correlates with delayed cerebral energy failure following perinatal hypoxia-ischaemia in the newborn pig. Exp Brain Res 156, 20–26 (2004). https://doi.org/10.1007/s00221-003-1761-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00221-003-1761-5