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Effect of the blockade of μ1-opioid and 5HT2A-serotonergic/α1-noradrenergic receptors on sweet-substance-induced analgesia

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Abstract

Rationale

Sweet-substance-induced analgesia has been widely studied, and the investigation of the neurotransmitters involved in this antinociceptive process is an important way for understanding the involvement of the neural system controlling this kind of antinociception.

Objective

The aim of this study was to investigate the involvement of opioid and monoaminergic systems in sweet-substance-induced analgesia.

Methods

The present work was carried out in an animal model with the aim of investigating whether acute (24 h) or chronic (14 days) intake of a sweet substance, such as sucrose (250 g/l), is followed by antinociception. Tail withdrawal latencies in the tail-flick test were measured before and immediately after this treatment. Immediately after the recording of baseline values, independent groups of rats were submitted to sucrose or tap-water intake and, after chronic treatment, they were pretreated with intraperitoneal administration of (1) naltrexone at 0.5, 1, 2 or 3 mg/kg; (2) naloxonazine at 5, 10, 20 or 30 mg/kg; (3) methysergide at 0.5, 1, 2 or 3 mg/kg; (4) ketanserin at 0.5, 1, 2 or 3 mg/kg; or (5) physiological saline.

Results

Naltrexone and methysergide at two major doses decreased sweet-substance-induced analgesia after chronic intake of a sweet substance. These effects were corroborated by peripheral administration of naloxonazine and ketanserin.

Conclusions

These data give further evidence for: (a) the involvement of endogenous opioids and a μ1-opioid receptor in the sweet-substance-induced antinociception; (b) the involvement of monoamines and 5HT2A serotonergic/α1-noradrenergic receptors in the central regulation of the sweet-substance-produced analgesia.

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Acknowledgements

This work was sponsored by FAPESP (proc. 95/3604-4, 02/01496-5, 02/03705-0 and 03/12882-6) and FAEPA (proc. 537/95 and 355/2000). E.N. Segato, R. Kishi and R.L. de Freitas were recipients of scholarships from FAPESP (proc. 98/07416-6, 00/02204-2 and 01/03752-6, respectively). E.C.C. Rebouças is the recipient of a Master fellowship from SAEB. R.L. de Freitas is the recipient of a Master fellowship from FAPESP (03/05256-1). N.C. Coimbra is the recipient of a research fellowship from CNPq (proc. 300772/97-1). The authors are grateful to Valdir Mazzucato Jr. and Daoud Hibrahim Elias Filho for expert technical assistance. D.H. Elias-Filho is the recipient of a scholarship from FAPESP (TT2, proc. 02/01497-1). We thank Prof. L.M.L. Caliri Juzzo and Dr. Constance Oliver for reviewing the manuscript.

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Correspondence to N. C. Coimbra.

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Rebouças, E.C.C., Segato, E.N., Kishi, R. et al. Effect of the blockade of μ1-opioid and 5HT2A-serotonergic/α1-noradrenergic receptors on sweet-substance-induced analgesia. Psychopharmacology 179, 349–355 (2005). https://doi.org/10.1007/s00213-004-2045-x

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  • DOI: https://doi.org/10.1007/s00213-004-2045-x

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