Table 1

First and second round fusion gene primers and breakpoint specific SSO probes

	5′–3′ sequence	Specificity	Location	Ref
Primer
TELS841	ATC ATG CAC CCT CTG ATC CT	TEL-AML1	5′ external	21
AMLAS765	TGG GCA GGG TCT TGT TGC AG	TEL-AML1	3′ external	21
B12	CGT GGA TTT CAA ACA GTC CA	TEL-AML1	5′ internal	24
AM3	CTC GCT CAT CTT GCC TGG	TEL-AML1	3′ internal	24
MLLAF4A	AGA GCA GAG CAA ACA GAA	MLL-AF4	5′ external	32
MLLAF4B	GCT GAG AAT TTG AGT GAG	MLL-AF4	3′ external	32
MLLAF4C	AAG TGG CTC CCC CGC CCA AGT AT	MLL-AF4	5′ internal	32
MLLAF4D	TTG GGT TAC AGA ACT GAC ATG	MLL-AF4	3′ internal	32
BCRA	CGG GAG CAG CAG AAG AAG TC	BCR-ABL b2a2 /b3a2	5′ external	33
BCRC	TTG TCG TGT CCG AGG CCA CC	BCR-ABL e1a2	5′ external	33
ABLA	TGT GAT TAT AGC CTA AGA CCC GGA G	BCR-ABL b2a2 /b3a2/e1a2	3′ external	33
BCRB	GTG AAA CTC CAG ACT GTC CAC AGC A	BCR-ABL b2a2 /b3a2	5′ internal	33
BCRD	CAA GAC CGG GCA GAT CTG GCC C	BCR-ABL e1a2	5′ internal	33
ABLB	TCC ACT GGC CAC AAA ATC ATA CAG T	BCR-ABL b2a2 /b3a2/e1a2	3′ internal	33
β-ACTIN 5′	TGA AGT CTG ACG TGG ACA TC	β-ACTIN 5′	5′
β-ACTIN 3′	ACT CGT CAT ACT CCT GCT TG	β-ACTIN 3′	3′

Probe
TEL-AML1 P1	AGA ATA GCA GAA TGC ATA CTT	Probe 267bp transcript TEL-AML1		31
TEL-AML1 P2	GAA TAG CAG ATG CCA GCA C	Probe 228bp transcript TEL AML1		31
AF4 P	TAG GGA AAG GAA ACT TGG ATG	Probe MLL-AF4		32
b2a2 P	GCT GAA GGG CTT CTT CCT TAT TGA TG	Probe b2a2 transcript BCR-ABL		33
b3a2 P	GCT GAA GGG CTT TTG AAC TCT GCT TA	Probe b3a2 transcript BCR-ABL		33
e1a2 P	GCT GAA GGG CTT CTG CGT CTC CAT	Probe e1a2 transcript BCR-ABL		33