Platelet immunology
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Genotyping: |
Maternal platelet genotype: | HPA-1b1b; HPA-2a2a; HPA-3a3b; HPA-4a4a; HPA-5a5b |
Paternal platelet genotype: | HPA-1a1b; HPA-2a2a; HPA-3a3a; HPA-4a4a; HPA-5a5a |
Twin 1 platelet genotype: | HPA-1b1b; HPA-2a2a; HPA-3a3b; HPA-4a4a; HPA-5a5a |
Twin 2 platelet genotype: | HPA-1a1b; HPA-2a2a; HPA-3a3a; HPA-4a4a; HPA-5a5b |
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Indirect platelet immunofluorescence (PIFT): |
IgG platelet antibodies detected in mother's serum |
Positive reactions with 6/6 HPA/HLA typed panel cells |
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Monoclonal antibody specific immobilisation of platelet antigen assay (MAIPA): |
Anti-HPA 1a antibody detected, titre >1/16 |
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Solid phase ELISA (PAK): |
Anti-HPA 1a antibody detected, HLA antibodies also detected |
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Crossmatch of maternal serum versus paternal platelets: |
Positive reactions between mother's serum and father's platelets |
Strongly positive for anti-GPIIb/IIIa |
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HLA typing and antibody screen
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Class I typing: |
Mother: | A1; B8 |
Father: | A2; B27 B60 |
Twin 1: | A1 A2; B8 B60 |
Twin 2: | A1 A2; B8 B27 |
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Lymphocyte cytotoxicity test (LCT): |
Maternal serum: | Multispecific HLA antibodies detected, titre >1/16 |
Infectious screen: |
Blood cultures: | Negative |
Urine cultures: | Negative |
TORCH: | Negative |