Macroscopic agglutination
| Sample | NS | D5W | 0.5% NS | IAA | IAA + cysteine | Isotonic sodium acetate | TPN | IAA + cysteine + minerals and vitamins | D5 0.25% NS | 0.25% NS |
| H | − | 2+ | − | − | 3+ | − | − | 1+ | − | Complete haemolysis |
| I | − | 2+ | − | − | 3+ | − | − | +/− | − | Complete haemolysis |
| J | − | 1+ | − | − | 2+ | − | − | +/− | − | Complete haemolysis |
| K | − | +/− | − | − | 3+ | − | − | − | − | Complete haemolysis |
| L | − | +/− | − | − | 3+ | − | − | 2+ | − | Complete haemolysis |
One drop of sample whole blood was mixed with 4 ml test solution, and the amount of macroscopic agglutination recorded; large clumps are classified as 4+, smooth appearance is classified as −, with +/−, 1+, 2+, 3+ being intermediate.9 ,11
NS, normal saline; D5W, isotonic dextrose; IAA, isotonic amino acids; TPN, total parenteral nutrition; D5 0.25% NS, 5% dextrose in 0.25% normal saline.