C5a and fMLP mediated exocytosis of azurophil and specific granules from newborn and adult PMN
| Newborns (n=20) | Adults (n=20) | ||||||
|---|---|---|---|---|---|---|---|
| ng/10−7 cells (SD) | Release percentage (SD) | ng/10−7 cells (SD) | Release percentage (SD) | ||||
| C5a triggered exocytosis of MPO | 438 (191)1-150 | 1.2 (0.7) | 602 (258)† | 1.5 (0.5) | |||
| fMLP triggered exocytosis of MPO | 1861 (937) | 5.2 (3.4) | 632 (258) | 1.6 (0.5) | |||
| C5a triggered exocytosis of LF | 657 (475)‡ | 7.1 (5.7) | 694 (225)** | 4.0 (1.5) | |||
| fMLP triggered exocytosis of LF | 1979 (1085) | 22.0 (13.6) | 1039 (409) | 5.8 (2.2) | |||
↵1-150 Total MPO = 40.1 μg/10−7 cells; † total MPO = 39.8 μg/10−7 cells; ‡ total LF = 10.7 μg/10−7 cells; total LF = 20.1 μg/10−7cells.
The exocytosis was calculated as amount of enzyme released after subtraction of the spontaneous enzyme release and expressed in ng/10−7 cells.
The percentage of total cell content is calculated by dividing the enzyme release with the total cell content measured after sonication of the PMN.
n=8 for all experiments.