Article Text

other Versions

PDF
Near infrared reflectace analysis to evaluate human milk's nitrogen and fat content in neonatal-intensive care unit
  1. Luigi Corvaglia (luicorva{at}almadns.unibo.it)
  1. Istituto Clinico di Pediatria Preventiva e Neonatologia - Osp. Sant'Orsola-Malpighi - Univ. Bologna, Italy
    1. Barbara Battistini
    1. Istituto Clinico di Pediatria Preventiva e Neonatologia - Osp. Sant'Orsola-Malpighi - Univ. Bologna, Italy
      1. Vittoria Paoletti
      1. Istituto Clinico di Pediatria Preventiva e Neonatologia - Osp. Sant'Orsola-Malpighi - Univ. Bologna, Italy
        1. Arianna Aceti
        1. Istituto Clinico di Pediatria Preventiva e Neonatologia - Osp. Sant'Orsola-Malpighi - Univ. Bologna, Italy
          1. Maria Grazia Capretti
          1. Istituto Clinico di Pediatria Preventiva e Neonatologia - Osp. Sant'Orsola-Malpighi - Univ. Bologna, Italy
            1. Giacomo Faldella
            1. Istituto Clinico di Pediatria Preventiva e Neonatologia - Osp. Sant'Orsola-Malpighi - Univ. Bologna, Italy

              Abstract

              Objective: To validate Near-Infrared Reflectance Analysis (NIRA) as a fast, reliable and suitable method for routine evaluation of human milk's nitrogen and fat content.

              Setting: One Neonatal Intensive Care Unit.

              Patients: 124 samples of expressed human milk (55 from preterm mothers and 69 from term mothers).

              Intervention: Measurement of nitrogen and fat content both by NIRA and traditional methods (Gerber method for fat and Kjeldhal method for nitrogen).

              Main outcome measures: Agreement between NIRA's and traditional methods' results. Variability in fat and nitrogen human milk content.

              Results: A strong agreement was found between traditional methods' and NIRA's results (expressed as g/100 g of milk), both for fat and nitrogen content in term (mean fat content: NIRA=2.76; Gerber=2.76; mean nitrogen content: NIRA=1.88; Kjeldhal =1.92) and preterm (mean fat content: NIRA=3.56; Kjeldhal=3.52; mean nitrogen content: NIRA=1.91; Kjeldhal =1.89) mother's milk. <BR>Nitrogen content of the milk samples, measured by NIRA, ranged from 1.18 to 2.71 g/100 g of milk in preterm milk and from 1.48 to 2.47 in term milk; fat content ranged from 1.27 to 6.23 g/100 g of milk in preterm milk and from 1.01 to 6.01 g/100 g of milk in term milk.

              Conclusions: NIRA can be used as a fast, reliable and suitable tool for routine monitoring of macronutrient content of human milk and for individualized human milk fortification in the feeding of very premature infants.

              • Human milk nitrogen and fat content
              • Near-Infrared Reflectance Analysis
              • Neonatal Intensive Care Unit

              Statistics from Altmetric.com

              Request permissions

              If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

              Linked Articles

              • Fantoms
                Martin Ward Platt