Article Text


Magnetic resonance imaging of the infant brain: anatomical characteristics and clinical significance of punctate lesions
  1. L G Cornette1,
  2. S F Tanner2,
  3. L A Ramenghi1,
  4. L S Miall1,
  5. A M Childs1,
  6. R J Arthur3,
  7. D Martinez3,
  8. M I Levene1
  1. 1Department of Paediatrics and Child Health, Leeds General Infirmary, Leeds, UK
  2. 2Department of Medical Physics and Centre of Medical Imaging Research, University of Leeds and Leeds General Infirmary
  3. 3Department of Paediatric Radiology, Leeds General Infirmary
  1. Correspondence to:
    Dr Cornette, Peter Congdon Neonatal Intensive Care Unit, Clarendon Wing C Floor, Leeds General Infirmary, Leeds LS2 9NS, UK;


Objective: To describe the magnetic resonance imaging (MRI) characteristics of punctate brain lesions in neonates (number, appearance, distribution, and association with other brain abnormalities) and to relate them to neurodevelopmental outcome.

Methods: A retrospective analysis was performed of 110 MRI brain scans from 92 infants admitted in 1998 to the neonatal intensive care unit. Results of routine neurodevelopmental follow up (1998–2001) in those infants with punctate brain lesions were analysed.

Results: Punctate lesions were observed in 15/50 preterm and 2/42 term infants. In the preterm group, the number of lesions was < 3 in 20%, 3–10 in 27%, and > 10 in 53%. In 14/15 the lesions were linearly organised and located in the centrum semiovale. Other brain abnormalities were absent or minor—that is, “isolated” punctate lesions—in 8/15 and major in 7/15. In the term group, punctate lesions were organised in clusters and no other brain abnormalities were observed. Isolated punctate lesions were observed in 10/17 infants, and a normal neurodevelopmental outcome was seen in 9/10 (mean follow up 29.5 months). One infant showed a slight delay in language development. In the infants with associated brain lesions (7/17, mean follow up 27.5 months), outcome was normal in only two subjects.

Conclusions: Punctate lesions are predominantly seen in preterm infants, are usually linearly organised, and border the lateral ventricles. Isolated punctate lesions may imply a good prognosis, because most of these subjects have a normal neurodevelopmental outcome so far.

  • magnetic resonance imaging
  • brain
  • punctate lesion
  • prognosis
  • MRI, magnetic resonance imaging
  • CUS, cranial ultrasound
  • PVL, periventricular leucomalacia
  • SI, signal intensity
  • GA, gestational age

Statistics from

The use of magnetic resonance imaging (MRI) has been successfully extended to the neonatal period over the last decade. At present, MRI data on normal brain development and on a wide range of pathological entities have been collected in both preterm and term infants.1 Whereas cranial ultrasound (CUS) has been pivotal in facilitating our understanding of severe white matter injury in the premature infant, such as cystic periventricular leucomalacia (PVL) and intraparenchymal infarctions, optimisation of MRI methods provides us with a finer anatomical resolution, allowing the detection of more discrete abnormalities.2 Punctate white matter hyperintensities are a common MRI observation in the elderly, and, according to histopathological correlations, correspond to focal rarefaction of myelin, loss of fibres, or lacunar infarctions.3 Only anecdotal reports exist on similar subtle white matter lesions in infants presenting with perinatal asphyxia4–6 or seizures.7 These lesions vary from hyperintense to hypointense on T2 weighted images and have been interpreted as focal areas of necrosis or haemorrhage. Using MRI, we also recently observed punctate white matter abnormalities in newborns.8 These lesions are < 5 mm in diameter and consist of an increased signal intensity (SI) on T1 weighted MR images—that is, T1 shortening—and decreased SI on T2 weighted images—that is, T2 shortening. So far, punctate brain lesions in the neonatal age group have not been studied systematically, and their “natural clinical outcome” has remained largely unknown. Hence, the aim of this study was to systematically describe the anatomical characteristics and clinical significance of punctate MRI brain lesions in a large cohort of preterm and term neonates admitted to our neonatal intensive care unit. We analysed all brain MRI scans performed in 1998 to allow at least two years clinical follow up.



In 1998, 837 infants were admitted to the neonatal unit of the Leeds General Infirmary (507 infants inborn and 330 infants referred from other hospitals). The unit accepts infants for intensive, medium, and special care (levels of care categories recommended by the British Association of Perinatal Medicine). Of all 1998 admission days, 21% were categorised as intensive care days. Recruitment for brain MRI was predominantly performed within this group of infants needing intensive care. We obtained consent in 109 infants, the scans of which were analysed retrospectively.

Eighty six of these infants were scanned for clinical indications, such as perinatal asphyxia (n = 24), convulsions with causes other than asphyxia (meningitis/encephalitis, MCA infarction, fits of unknown origin, n = 21), congenital or posthaemorrhagic hydrocephalus (with or without complications such as ventriculitis, n = 16), dysmorphism with or without brain malformation on CUS and/or chromosomal abnormality (n = 14), (severe) PVL (n = 7), persistent echodensities on CUS (n = 2), congenital infection (n = 1), and mitochondrial disorder (n = 1).

The remaining 23 infants all had intravenous access in situ at the time that they were well enough to undergo MRI and were therefore recruited for another continuing MRI research project, measuring brain perfusion. No definite abnormalities were observed on CUS in this group. Although there was no specific clinical indication in these infants for brain MRI, 15 of these 23 babies were given intensive care in the neonatal period.

Anatomical MRI details were obscured by movement artefact in 17 subjects. Therefore 92 of the subjects were included for full MRI analysis (48 boys, 44 girls; 54 inborn, 38 referred; 71 scanned for clinical reasons, 21 for research purposes). Informed consent for the scan was obtained on all occasions and the MRI procedure was approved by the research ethics committee of the Leeds General Infirmary.

We classified the 92 subjects into two different groups: (1) preterm infants (gestational age (GA) < 37 weeks, n = 50); (2) term infants (GA ≥ 37 weeks, n = 42). The median birth weight of the preterm infants was 1280 g (SD 739, range 580–3675) for a median GA of 30 weeks (SD 3.5, range 25–36). The median birth weight of the term infants was 3160 g (SD 715, range 2035–4430) for a median GA of 40 weeks (SD 1.4, range 37–41). Median age at MRI in the preterm group was 13 days (SD 24, range 2–106). Median age at MRI in the term group was 14 days (SD 64, range 2–273).

A repeat scan was performed only when further characterisation of an observed MRI abnormality was essential as part of clinical management, and was obtained in 18 of the subjects (12 preterm and six term infants).

MRI acquisition

All infants underwent the same imaging protocol on a 1.5 T Gyroscan ACS-NT (Philips Medical Systems, Eindhoven, The Netherlands), with a receive only quadrature head coil. Firstly, T1 weighted spin echo images were acquired in the sagittal and axial planes. The acquisition parameters used to acquire the T1 weighted images were: repetition time 800 milliseconds; echo time 13 milliseconds; field of view 180 mm; slice thickness 4 mm with a 0.4 mm gap; acquisition matrix 256 × 256; scan time 3 min 52 seconds. Secondly, T2 weighted fast spin echo images were acquired in the axial plane. The parameters used were: repetition time 6000 milliseconds; echo time 200 milliseconds; echo train length 13; field of view 180 mm; slice thickness 3 mm with a 0.3 mm gap; acquisition matrix 256 × 256; scan time 5 min 12 seconds. Thirdly, because of its superior sensitivity to acute injury, MRI diffusion measurements were attempted when evidence obtained from the T1 and T2 weighted images indicated that the infants were not moving. An orientation independent measure of the apparent diffusion coefficient was obtained by calculating the average of three orthogonal diffusion measurements.9 The protocol consisted of a multishot spin echo echoplanar imaging sequence that was electrocardiographically triggered. A navigator echo was used to reduce motion artefacts. The parameters used were echo time 105 milliseconds, slice thickness 6 mm with a 1 mm gap, field of view 180 mm, and acquisition matrix 256 × 256.

All scans were performed after a feed, and most infants were imaged without the use of sedation. If necessary, subjects were sedated with 30–50 mg/kg chloral hydrate administered orally. They were continuously monitored by pulse oximetry and electrocardiographic recording. Mechanical ventilation, continuous positive airway pressure, and administration of intravenous fluids and inotropic drugs were continued if necessary. Blankets wrapped around the babies kept them warm during scanning, and cotton wool placed in their ears diminished the effects of gradient generated noise. The head was immobilised by a pillow filled with polystyrene balls and moulded into shape by vacuum extraction. This reduced image artefacts arising from head movement. A paediatrician was present during the transfer of babies between the neonatal unit and the scanner, and throughout the MRI study.

MRI analysis

The MRI scans were viewed on a Philips Easyvision stand alone workstation, using proprietary software, by four trained investigators (LGC, LSM, RJA, DM) blinded to the clinical data of study participants. A standardised system of visual assessment was used to describe each scan, and consensus was reached for each infant. Punctate lesions were defined as spots (< 5 mm) of increased SI on T1 weighted images and decreased SI on T2 weighted images. Features recorded included (a) number of punctate lesions (categorised into < 3, 3–10, > 10), (b) appearance of the lesions (organised in clusters—that is, a confluent pattern—, linearly or mixed-type pattern), and (c) location (anterior to the frontal horn of the lateral ventricles (anterior region), posterior to the occipital horn of the lateral ventricles (posterior region), or in between (mid-region or centrum semiovale); and observed unilaterally or bilaterally). This location strategy was based on our expectation that anterior located lesions would remain asymptomatic in a neonate, because the anterior frontal cortex shows little metabolic activity at this stage of development.10 In contrast, a lesion located in the centrum semiovale may cause motor dysfunction during follow up. In addition, possible association with other brain abnormalities defined as minor (cortical highlighting, intraventricular haemorrhage grade I or IIa,11 discrete oedema) or major (intraventricular haemorrhage grade IIb or III,11 venous infarction zones, cystic PVL, extensive cortical atrophy, and ventricular dilatation) was investigated.

Follow up strategy

All infants admitted to our neonatal unit are subjected to regular clinical follow up, at either Leeds General Infirmary or the referring hospital. Our policy is to follow up all inborn infants who received intensive care, until they reach the age of two years. Infants referred from other centres are normally followed up at the referring hospital, unless under the care of a tertiary specialist. The infants are seen at regular intervals (three to six months). Neurodevelopmental assessment focuses on the achievement of developmental milestones, using standard questions in the gross motor, person-social, language, and fine motor-adaptive realms. Apart from a general physical examination, the neurological examination involves careful examination of the skull, cranial nerves, posture, muscle tone, reflexes, power, coordination, and sensory system. All neonatologists involved are experienced in standard clinical neurological examination and forward their observations on a regular basis to the consultants at Leeds General Infirmary.


MRI analysis in preterm infants (n = 50)

Punctate abnormalities were observed in 15/50 (10 boys, five girls) preterm infants (table 1A,B). The median birth weight was 1320 g (SD 929, range 580–3960), and the median GA 31 weeks (SD 2.9, range 27–36). Median age at MRI was 16 days (SD 20.4, range 2–82). The median postconceptional age at MRI was 35 weeks (SD 3.1, range 29–39.7). Individual details of antenatal (maternal systemic diseases, obstetric risk factors, intrauterine growth retardation) and perinatal (type of delivery, Apgar scores) events are summarised (table 1). Except for premature delivery, pregnancy was uneventful in six. Six subjects were delivered by emergency caesarean section for fetal distress, and all 15 subjects needed intensive care during the first week(s) of life.

Table 1

Summary of infants with punctate lesions

All punctate lesions were located in the white matter. The number of lesions was < 3 in three (all clusters), 3–10 in four (two linearly organised, two mixed-type pattern), and > 10 in eight (six linearly organised). The lesions were seen in the mid (n = 14), posterior (n = 8), and anterior (n = 3) regions of the brain. All clusters were localised in one hemisphere, whereas all linear or mixed-type lesions were observed bilaterally. In four babies, no other brain abnormality was seen (fig 1A), in four babies punctate lesions were associated with minor abnormalities (discrete oedema), and in seven babies there were other major lesions (cystic PVL, left cerebellar haemorrhage, infarction in the basal ganglia, and herpetic encephalitis; fig 1B). In one subject with cystic PVL, a repeat scan performed seven days after the first one showed that the punctate lesions had disappeared. No punctate lesions were identified in 35/50 (22 girls, 13 boys) preterm infants. Within this group, no brain abnormalities were observed in 18, minor abnormalities were seen in four, and major abnormalities in 13. The other abnormalities in the 13 subjects included loss of parenchymal detail because of extensive cyst formation or gross posthaemorrhagic hydrocephalus. No congenital brain malformations were seen.

Figure 1

Magnetic resonance characterisation of punctate lesions. (A) Isolated punctate lesions in an infant of 28 weeks gestation, scanned at 7 days of age (corresponding to case 1 in table 1). Left panel: T1 weighted axial image through the body of the lateral ventricles. Bilateral high intensity spots (arrows) correspond to punctate lesions, predominantly seen in the mid and posterior regions of the brain. In the right hemisphere, lesions (about 10) are linearly organised in the periventricular white matter. In the left hemisphere, lesions are organised in a mixed-type pattern (clustered and linear). No other brain abnormalities are observed. L, Left. Right panel: corresponding T2 weighted axial image of same infant. Bilateral low intensity spots (arrows) correspond to punctate lesions. (B) Punctate lesions in an infant of 29 weeks gestation with cystic periventricular leucomalacia (PVL), scanned at 26 days of age (corresponding to case 11 in table 1). Left panel: T1 weighted axial image through the body of the lateral ventricles. Bilateral high intensity spots (arrows) correspond to punctate lesions, predominantly seen in the mid and posterior regions of the brain. In the two hemispheres, the lesions are linearly organised and border a medially located discrete band of low signal intensity lesions in the periventricular white matter. Right panel: corresponding T2 weighted axial image of same infant. Bilateral low intensity spots (arrows) correspond to punctate lesions. The punctate lesions are clearly neighbouring a medial region of cystic PVL (high intensity lesions, corresponding to cystic degeneration). (C) Isolated punctate lesions in an infant of 40 weeks gestation, scanned at 51 days of age (corresponding to case 16 in table 1). Left panel: T1 weighted axial image through the body of the lateral ventricles. Bilateral high intensity spots (arrows) correspond to punctate lesions, exclusively seen in the anterior region of the brain. No anatomical or myelination abnormalities are observed. Right panel: corresponding T2 weighted axial image of same infant. Low intensity spots (arrow) correspond to punctate lesions. In the right hemisphere, a cluster of six lesions is observed, whereas only one spot is clearly distinguished on the T1 weighted image.

MRI analysis in term infants (n = 42)

Punctate abnormalities were seen in only two of the term infants (both girls, GA = 40 weeks, birth weights 3025 and 3055 g; table 1C). In both subjects, the lesions were organised in clusters. In one subject, we observed > 10 punctate lesions, present in both hemispheres (fig 1C), while in the other < 3 lesions were observed, confined to the left hemisphere. The latter subject had also suffered a subarachnoid haemorrhage. Interestingly, a further scan four weeks later indicated that both the haemorrhage and punctate lesions had completely disappeared. No congenital brain malformations were seen in any of the term subjects.

MRI diffusion measurements

We acquired average diffusion weighted images in five of the ten babies (eight preterm and two term) with isolated punctate lesions (no or only minor other brain lesions). The diffusion weighted images did not show any corresponding abnormalities.


Figure 2 summarises all characteristics of the lesions. We divided the cohort (n = 92) into three subgroups: (a) GA < 30 weeks (n = 19), (b) GA between 30 and 36 weeks (n = 31), and (c) GA ≥ 37 weeks (n = 42). Babies with more than 10 lesions were predominantly observed in groups a and b; 3–10 lesions were observed only in group b; < 3 lesions were observed across all three groups (fig 2A). Predominantly linear lesions were observed in preterm infants with punctate lesions (groups a and b), followed by a cluster-type pattern; a mixed-type pattern was seen in only three subjects (fig 2B). Most of the cases were observed in the mid region of the brain, present across all preterm ages; fewest lesions appeared in the anterior region (fig 2C).

Figure 2

Overview of punctate lesions. Results are grouped according to the gestational age of all subjects analysed (n = 92): < 30 weeks (n = 19), between 30 and 36 weeks (n = 31), and ≥ 37 weeks (n = 42). (A) Number of punctate lesions. The percentage of cases with < 3, 3–10, or > 10 lesions is plotted for the different groups. (B) Appearance of punctate lesions. The percentage of cases with linear, clustered, or mixed appearance is plotted for the different groups. (C) Topography of punctate lesions. The percentage of cases in the anterior, mid, or posterior region of the brain is plotted for the different groups. As lesions were observed in the anterior, mid, and posterior region of the brain, we multiplied the total number of cases by three to calculate the percentage.

Neurodevelopmental outcome

No subjects were lost to follow up during the study. The mean follow up time of all preterm and term infants with isolated punctate lesions (n = 10) is currently 29.5 months (SD 3.5, range 23.2–34.1). So far, their neurodevelopmental outcome is favourable (table 1A,C): no subject died, no motor impairments have been noted, and only a slight delay in language development was seen in one girl at the age of 34.1 months. The clinical outcome of subjects with punctate lesions and other major lesions—that is, all preterm born infants (n = 7)—is less favourable (table 1B). The mean follow up time in this group is currently 27.5 months (SD 3.9, range 24.4–34.8). So far, clinical evaluation is normal in the two subjects with a haemorrhage, while cerebral palsy (spastic diplegia or quadriplegia) developed in three subjects with cystic PVL and in the subject with herpetic encephalitis. One subject with severe cystic PVL died at the age of 3.5 weeks. Hence, outcome in the latter group seems to be largely dependent on the severity and extent of the associated non-punctate lesions.


In this study, we identified the MRI characteristics of punctate brain lesions in a large cohort of neonates and infants (n = 92). The lesions were identified on the basis of focal increases (T1 weighted) and decreases (T2 weighted) in SI. In addition, we related the lesions to neurodevelopmental outcome.

MRI characteristics of punctate brain lesions

This study was not designed as an epidemiological survey of the prevalence of white matter abnormalities, as we studied a cohort of preterm and term babies, with and without clinical problems. Although we do not imply that punctate lesions are a common condition across all infants, our study indicates that they are not uncommonly observed in infants requiring neonatal intensive care. In the subjects analysed, they predominantly occurred in preterm infants (88%), the youngest infant having a postconceptional age of 29 weeks at the time of the scan. In most subjects, we observed > 10 discrete punctate lesions, although in subjects with associated brain damage the number of punctate lesions may have been underestimated because of extensive colateral tissue damage. In the most preterm group (postconceptional age < 33 weeks), the lesions were most commonly linearly organised, whereas in older infants (postconceptional age > 33 weeks), lesions were organised more in clusters. Most lesions were observed in the mid region of the brain—that is, bordering the lateral ventricles in the centrum semiovale.

The punctate lesions described in this study clearly differ from at least three other subtle changes in SI observed in the frontal white matter of infants. Firstly, they differ from the subtle structures of reduced SI observed bilaterally in the frontal periventricular white matter of the premature brain (T2 weighted images). These more band-like structures reflect the normal brain maturation phenomenon of groups of glial cells migrating to the cortex.6,12 Secondly, the currently described punctate lesions are different from the symmetrical focal areas of high T1 weighted and low T2 weighted SI that are restricted to the frontal horns of both hemispheres and which are believed to correspond to remnants of the germinal matrix.13 Thirdly, their SI is clearly different from frontal white matter punctate pseudolesions.14 These lesions have a high SI on T2 weighted images and are thought to occur because of a locally decreased myelin content, increased periependymal water collection, and ependymitis granularis.

Are punctate brain lesions petechial haemorrhages?

One should be cautious in inferring the exact nature of punctate lesions in infants. Indeed, no histological specimen was available in our cohort of infants, because parents of the infant with cystic PVL who died did not give consent for a postmortem examination. However, there are a number of reasons that make us believe punctate lesions correspond to petechial or focal areas of haemorrhage. Firstly, a repeat MRI scan, although obtained in only two subjects with established lesions, indicates that punctate lesions probably do not reflect local areas of permanent tissue destruction because they disappeared in both cases. Secondly, the fact that the lesions were seen slightly more clearly on T2 weighted images than on T1 weighted images corresponds to the observation that haemoglobin degradation products cause T2 shortening before they cause T1 shortening.15 Other causes of T1 and T2 shortening have been suggested, including haemosiderin deposition,4 dystrophic calcification, and presence of lipids from myelin breakdown.16 These are less likely because of the seemingly short lived and benign nature of the punctate lesions in the current study group. Interestingly, the predominantly linear organisation of isolated punctate lesions in preterm infants, bordering the lateral ventricles and involving particularly the centrum semiovale, corresponds to the distribution of PVL injury seen in preterm infants.17 Cystic PVL was the most commonly observed associated lesion. It is uncertain whether the punctate lesions are part of the pathophysiology of PVL, as they were also observed in two term infants, although in the anterior part of the brain. However, discrete haemorrhage into PVL lesions has been described in autopsy specimens and is, most commonly, petechial and circumscribed in nature.18 As lesions smaller than 5 mm are largely missed by CUS,4 one could argue that, at least in the premature infants, the punctate lesions may correspond to PVL grade I—that is, a transient hyperechogenicity of white matter that resolves without cyst formation.19 As most of the current MR images were acquired later than the CUS images, we did not compare the two imaging modalities in detail. At least in the isolated cases of punctate lesions, no obvious changes in echodensity were observed in the available CUS images. However, it is possible that, for each abnormal finding, there is a different optimal time for identification.16 In addition, the diffusion weighted images did not show any evidence of corresponding petechial abnormalities. Although MRI diffusion measurements in neonates are known to be sensitive to hypoxic ischaemic encephalopathy, the appearance of abnormalities depends on the relative timing of the MRI examination and the ischaemic insult.20 The failure to detect punctate abnormalities in diffusion data may therefore relate to the timing of the MRI examination, the large voxel sizes (1.4 mm × 1.4 mm × 6 mm) used, and/or the relatively low levels of signal intensity obtained.

Clinical significance of punctate brain lesions

In elderly normal subjects, a recent three year follow up study of these punctate lesions did not indicate an association between punctate lesions and cognitive functioning.3 In infants with large lesions such as cerebral infarctions, it has been shown that the detection of an abnormal sign during early neonatal neurological examination is a poor prognostic indicator.7 So far, punctate brain lesions in the neonatal age group have not been studied systematically. Early and follow up MRI studies in either premature or term infants with perinatal brain damage are, however, useful tools in predicting early and late clinical outcome.7,21–25 In our study, we carefully monitored neurodevelopment of all infants with isolated punctate brain lesions, which was normal in most (9/10, mean follow up 29.2 months). This favourable outcome was not introduced by subject selection bias, as intensive care treatment—for example, mechanical ventilation, resuscitation, or gastro-intestinal surgery—was needed in all infants. Hence, one can be optimistic when counselling parents about the risk of their babies having an abnormal neurological outcome. This is in agreement with the observation that minor CUS abnormalities identified within the first 96 hours of life, such as a mild ventricular enlargement, choroid plexus cyst, or subependymal cyst, mostly have no clinical significance in the immediate neonatal period, although they may cause several subtle abnormalities in behaviour beyond the neonatal period.26 On the other hand, subtle neurological disturbances such as asymmetry in tone or reflexes can be difficult to demonstrate in neonates and infants.27

Although we are currently expanding the cohort of infants studied by investigating all MRI brain scans performed after 1998, this report discusses only those performed in 1998, because it provided us with at least two years of clinical follow up. We accept that two years of follow up is still too short to determine whether or not punctate lesions can predict a more subtle clinical deficit. In time, it may be that the largely “asymptomatic” punctate brain abnormalities, already detectable on MRI scans during neonatal life, will be associated with mild cognitive deficits at school age. Therefore, we plan a thorough neuropsychological evaluation in all 10 children at the age of 5 (Wechsler preschool and primary scale of intelligence to assess cognitive functioning; revised and movement ABC to assess motor functioning). Because of the rapid advances currently obtained in MR image resolution through the use of head coils specifically adapted for infants or children and the improving resolution of diffusion weighted imaging,28,29 this neuropsychological evaluation will be combined with a repeat MRI investigation. The true significance of punctate brain lesions will only become apparent once there has been a sufficiently long period of follow up.


We are much indebted to all staff of the neonatal unit and the MRI department for their assistance. This work was supported by a grant from the special Trustees of the Leeds General Infirmary and from the Rotary Foundation. LC is a Consultant Neonatologist.


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