ORIGINAL ARTICLE

Association of interferon T⁺⁸⁷⁴A and interleukin 12 p40 promoter CTCTAA/GC polymorphism with the need for respiratory support and perinatal complications in low birthweight neonates

G Bokodi¹, L Derzbach¹, I Bányász¹, T Tulassay², B Vásárhelyi²

¹ First Department of Pediatrics, Semmelweis University, Budapest, Hungary
² Research Group of Pediatrics and Nephrology, Hungarian Academy of Sciences, Budapest, Hungary

Correspondence to:
G Bokodi
First Department of Pediatrics, Semmelweis University Budapest, Bókay u 53 H-1083, Hungary; bokodi{at}gyer1.sote.hu

Background: Data support the role of interferon (IFN) and interleukin (IL)12 in perinatal complications. IFN T⁺⁸⁷⁴A and IL12 p40 promoter CTCTAA/GC polymorphisms may have an effect on cytokine production.

Methods: DNA was extracted from dried blood samples of 153 low birthweight (LBW) infants and 172 healthy term infants. IFN and IL12 genetic polymorphisms were determined to investigate the association between polymorphisms and ventilation characteristics, bronchopulmonary dysplasia (BPD) and other perinatal disorders.

Results: The IFN⁺⁸⁷⁴A allele was over-represented in LBW infants. Carriers of the IFN⁺⁸⁷⁴T allele required mechanical ventilation and oxygen supplementation for time periods 41% and 35%, respectively, shorter than those required by those not carrying the IFN⁺⁸⁷⁴T allele. Stepwise logistic regression analysis showed that carriers of the IFN⁺⁸⁷⁴T allele were protected against BPD (odds ratio (OR) 0.35 (95% confidence interval (CI) (0.12 to 0.99))) and patent ductus arteriosus (OR 0.43 (95% CI 0.19 to 0.97)), whereas carriers of the IFN⁺⁸⁷⁴A allele were at higher risk of severe hypotension (OR 3.40 (95% CI 1.01 to 11.52)) and respiratory distress syndrome (OR 4.03 (95% CI 1.30 to 12.50)). Carriers of the IL12 GC allele were protected against pneumonia (OR 0.32 (95% CI 0.14 to 0.75)). Carriers of the IL12 CTCTAA allele were at higher risk of developing necrotising enterocolitis (NEC; OR 2.37 (95% CI 1.01 to 5.53)).

Conclusions: Carrier state of the IFN⁺⁸⁷⁴A allele presents an increased risk for premature birth and lung damage, as well as other perinatal complications. The risks of pneumonia and NEC are higher in heterozygotic carriers of the IL12 CTCTAA/GC polymorphism. Further studies are needed to determine whether these associations are the result of altered cytokine-producing capacity in infants carrying the tested alleles.

Abbreviations: BPD, bronchopulmonary dysplasia; IFN, interferon; IVH, intraventricular haemorrhage; IRDS, idiopathic respiratory distress syndrome; LBW, low birthweight; NEC, necrotising enterocolitis; PCR, polymerase chain reaction; PDA, patent ductus arteriosus; RFLP, restriction fragment length polymorphism; SNP, single-nucleotide polymorphism; TNF, tumour necrosis factor

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Henderson, G, Craig, S, Baier, R J, Helps, N, Brocklehurst, P, McGuire, W (2009). Cytokine gene polymorphisms in preterm infants with necrotising enterocolitis: genetic association study. Arch. Dis. Child. Fetal Neonatal Ed. 94: F124-F128 [Abstract] [Full Text]  
• Bhandari, V., Zhou, G., Bizzarro, M. J., Buhimschi, C., Hussain, N., Gruen, J. R., Zhang, H. (2009). Genetic Contribution to Patent Ductus Arteriosus in the Premature Newborn. Pediatrics 123: 669-673 [Abstract] [Full Text]  
• Bhandari, V., Gruen, J. R. (2007). The Genomics of Bronchopulmonary Dysplasia. NeoReviews 8: e336-e344 [Abstract] [Full Text]