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Cytomegalovirus remains viable in naturally infected breast milk despite being frozen for 10 days

¹ Paediatric Infectious Diseases Unit, Department of General Medicine, Royal Children’s Hospital, Melbourne, Australia
² Department of Paediatrics, University of Melbourne, Royal Children’s Hospital, Melbourne, Australia
³ Murdoch Children’s Research Institute, Melbourne, Australia
⁴ Department of Clinical Microbiology and Infectious Diseases, Royal Women’s & Royal Children’s Hospitals, Melbourne, Australia
⁵ Department of Obstetrics and Gynaecology, University of Melbourne, Royal Women’s Hospital, Melbourne, Australia
⁶ Virology Laboratory, Department of Microbiology and Infectious Diseases, Royal Children’s Hospital, Melbourne, Australia
⁷ Department of Neonatal Medicine, Royal Women’s and Royal Children’s Hospitals, Melbourne, Australia

Correspondence to:
Correspondence to:
Associate Professor Curtis
Department of Paediatrics, University of Melbourne, Royal Children’s Hospital, Parkville, VIC 3052, Australia; nigel.curtis{at}rch.org.au

ABSTRACT
Cytomegalovirus culture positive breast milk was obtained from four mothers of very premature babies. The milk was stored at 0–5°C in a domestic refrigerator for 48 hours or frozen for different durations at –20°C. Cytomegalovirus survived in breast milk despite being frozen for 10 days at –20°C.

Abbreviations: CMV, cytomegalovirus; RETCIF, rapid enhanced tissue culture immunofluorescence

Keywords: breast milk; cytomegalovirus; freezing; infection